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Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagExpressionMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterUASAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:Lamp1-mGFP
Plasmid#192356PurposeLamp1 tagged with mGFP, expressed under the UAS promoterDepositorInsertLamp1 (LAMP1 Human)
UseTagsmGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-PLK1-mCherry
Plasmid#127154PurposeExpresses mCherry PLK1 in mammalian cells and zebrafish embryosDepositorInsertPLK1-mCherry (PLK1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-Rab8b in pCSDEST2
Plasmid#140881PurposeExpression of zebrafish GFP-tagged Rab8b in mammalian cells.DepositorInsertRab8b (rab8b Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCS2-mCherry-Rab11a (Q70L)
Plasmid#184037PurposeExpresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationGlutamine 70 changed to LeucinePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5c-DN
Plasmid#109737PurposeMultisite gateway vector for 3' tagging with dominant negative rab5cDepositorInsertrab5c (rab5c Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCS2-mCherry-Rab11a (S25N)
Plasmid#184036PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationSerine 25 changed to AsparaginePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad2 (100-467)
Plasmid#14928DepositorInsertSmad2 (100-467) (SMAD2 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationamino acids 100-467PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad2 (1-185)
Plasmid#14929DepositorInsertSmad2 (1-185) (SMAD2 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationamino acids 1-185PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5c-CA
Plasmid#109738PurposeMultisite gateway vector for 3' tagging with constitutively active rab5cDepositorInsertrab5c (rab5c Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationconstitutively activePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLR2070
Plasmid#28063DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationContains N496D point mutation in FokI domain, as …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCLR2068
Plasmid#28062DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationContains H537R point mutation in FokI domain, as …PromoterAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a in pCSDEST2
Plasmid#140880PurposeExpression of zebrafish GFP-tagged Rab8a in mammalian cells.DepositorInsertRab8a (rab8a Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab32a
Plasmid#109689PurposeMultisite gateway vector for 3' tagging with rab32aDepositorInsertrab32a (rab32a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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