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Showing: 2821 - 2840 of 72239 results

  2. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193786-plasmid-map-sequence-id-389837

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  3. pODN-mNG-CfANLN

    Plasmid
    #183837
    Purpose
    Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    Canis familiaris ANLN homology arms with mNeonGreen-linker
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183837-plasmid-map-sequence-id-362449

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  4. TMD20

    Plasmid
    #163675
    Purpose
    Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163675-plasmid-map-sequence-id-322680

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  5. TMD21

    Plasmid
    #163676
    Purpose
    Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163676-plasmid-map-sequence-id-322681

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  6. TMD22

    Plasmid
    #163677
    Purpose
    Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163677-plasmid-map-sequence-id-322682

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  7. TMD24

    Plasmid
    #163678
    Purpose
    Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163678-plasmid-map-sequence-id-322683

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  8. Clim Actin MCH Puro (CAMP)

    Plasmid
    #104446
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Article
    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Tags
    Expression
    Mutation
    Promoter
    actin
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    104446-plasmid-map-sequence-id-200867

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  14. pcDNA3-EngPDm

    Plasmid
    #64040
    Purpose
    Mammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    Pax8 paired domain (Pax8 Mouse)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    paired domain, aa 1-147, with aa 30 to 32 changed…
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3-EngHDm

    Plasmid
    #64038
    Purpose
    Mammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    TTF-1 (TTF1 dog)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    homeodomain, aa 154-226, with aa 48–50 replaced b…
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2821 - 2840 of 72239 results