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Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-EGFP-L2
Plasmid#141046PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-nCas9n-P2A-TagBFP2-pA
Plasmid#176026PurposeMultisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENTDepositorInsertnCas9n-P2A-TagBFP2
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY273
Plasmid#184155PurposeReprogrammed short tracrRNA for hijacking mRNA of zraPDepositorInserts-tracrRNA-zraP-22
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF1-2A-EGFP-L2
Plasmid#141048PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF1-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2
Plasmid#141052PurposeMutisite Gateway mediated vector constructionDepositorInserteSpCas9(1.1)-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
gCBEv3
Plasmid#220618Purposevector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoterDepositorArticleInsertnSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
UseTagsExpressionMammalianMutationhUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLY232
Plasmid#184149Purposeshort tracrRNA generator with WT anti-repeat regionDepositorInserts-tracrRNA-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY283
Plasmid#184156PurposeReprogrammed short tracrRNA for hijacking mRNA of zntADepositorInserts-tracrRNA-zntA-1034
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-EGFP-L2
Plasmid#141045PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2
Plasmid#141051PurposeMutisite Gateway mediated vector constructionDepositorInserteSpCas9(1.1)-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-FokI-dCas9-L2
Plasmid#141042PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9n-EGFP-L2
Plasmid#141044PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9n-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-L2
Plasmid#141041PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9-EGFP-L2
Plasmid#141043PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF4-2A-EGFP-L2
Plasmid#141049PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF4-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF2-2A-EGFP-L2
Plasmid#141050PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF2-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only