Addgene

We narrowed to 6,704 results for: 11

Showing: 2821 - 2840 of 6704 results

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pCFJ1200

Plasmid

#44485

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a2(2nd methione)

Plasmid

#15747

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2(2nd methione) (MAPK10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5L18-NtermN1

Plasmid

#51444

Purpose

Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K150Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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ATF4 9: (SL+)5’ATF4.GFP

Plasmid

#21856

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem loop introduced at the 5' end of the mR…

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 7

Plasmid

#8481

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

F320S

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
myc GbetaL mutant 2

Plasmid

#8478

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S72D

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pMCh-1016

Plasmid

#92255

Purpose

Plasmid for expression of HA-tagged human TNRC6C CED in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

.C-terminal effector domain (CED): aa 1369-1690

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 C140S (#622)

Plasmid

#99947

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C140S

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-133

Plasmid

#92256

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

.C-terminal effector domain (CED): aa 1369-1690

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 1

Plasmid

#8477

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

D42A

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 6

Plasmid

#8480

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T208D

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

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pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 A. victoria, Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Promoter

Available since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

Plasmid

#73306

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Use

Tags

intein

Expression

Bacterial

Mutation

Q867E-Q939E-C935S-Q1011E-C1007S

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS2_HindIII

Plasmid

#65666

Purpose

FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1DCH2B

Plasmid

#13471

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2B; Delta 47-137

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5R18-NtermN1

Plasmid

#51445

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-NtermN2

Plasmid

#51446

Purpose

Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K150Q and K153Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TALEN-CCR5R18-NtermN2

Plasmid

#51447

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N2 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q and K153Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only