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cmv hTid Long

Plasmid

#13707

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long (DNAJA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

Plasmid

#67648

Purpose

I-SceI cleavage site at lys1-hphMX6

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

lys1 (lys1 Fission Yeast)
hphMX6

Use

To test i-scei cleavage efficiency

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMEH60 Lamp2b MS2 HA

Plasmid

#168209

Purpose

Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Lamp2b (LAMP2 Human)

Use

Tags

HA and MS2 dimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Pfba1-PYC-Trpl15a

Plasmid

#127738

Purpose

Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Jan. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

Plasmid

#101202

Purpose

Bacterial expression plasmid for SpCas9∆REC3 variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant M1–N497,GGS,V713–D1368

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

M1–N497, GGS, V713–D1368

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRS315-0nt-telo-I-SceI

Plasmid

#67624

Purpose

telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
telomere cassette and I-SceI cut site
hph

Use

Yeast cen/ars plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pHL 1917

Plasmid

#53024

Purpose

AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

mCherry
fimB (fimB E. coli)

Use

Synthetic Biology

Tags

Asp terminator, RBS(st7), and T1 terminator

Expression

Bacterial

Mutation

Promoter

pCon and pLlacO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-E6AP-3'UTR-miR-375-mut

Plasmid

#53695

Purpose

Luciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Tags

Expression

Mutation

Mutation on miR-375 binding site on E6AP 3'U…

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pDSM-ab-Psptef1-NAT-Ttip1

Plasmid

#127747

Purpose

Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

nourseothricin N-acetyltransferase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Psptef1

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3071

Plasmid

#41245

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ets1a-Right (ets1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
TAL3070

Plasmid

#41244

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ets1a-Left (ets1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
CMYA5T2/pDEST15

Plasmid

#98109

Purpose

Express CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

rs10043986 (NP_705838.3:p.Pro4063Leu)

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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TAL3403

Plasmid

#47122

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-apoeb-right (apoeb Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3402

Plasmid

#47121

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-apoeb-left (apoeb Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter hsa-mir17~20

Plasmid

#46671

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir17~20 (MIR17 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

Plasmid

#101213

Purpose

Bacterial expression plasmid for SpCas9 + K855A variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K855A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K855A

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3040

Plasmid

#41214

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-cebpa-Left (cebpa Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3041

Plasmid

#41215

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-cebpa-Right (cebpa Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3106

Plasmid

#41280

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-kinesin-family-member-13a-Left (kif13a Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only