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Plasmid#82589Purpose3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purificationDepositorInsertGFP-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2336
Plasmid#67541PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2woH
Plasmid#65018PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)
Plasmid#52007PurposeExpresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codonDepositorInsert5utr-MAVS-ORF2,3mut
UseTagsExpressionMammalianMutationmutated start codons for ORF3 and 4PromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Oncorhynchus kisutch cryaa
Plasmid#47667PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Pimephales notatus cryaa
Plasmid#47621PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dissostichus mawsoni cryaa
Plasmid#46167PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 2: AIRAP(m1.9-T7)pGEX.KG
Plasmid#21792DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B3.MJ0130m
Plasmid#11528DepositorInsertType I restriction-modification enzyme (MJ_0130m M. jannaschii)
UseTagsHisTag, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B4.TM1717
Plasmid#11532DepositorInsertYjeQ GDP binding protein (TM1717 T. maritima)
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
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p5E-EF1a/b-actin
Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
UseTagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEF-BFP
Plasmid#138272PurposeExpression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editorDepositorInsertBFP H66 PAM
UseCRISPRTagsExpressionMammalianMutationH66, PAM motifPromoterEf1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
UseTags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationFragmentPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 WT
Plasmid#199573PurposeExpresses FLAG-tagged WT SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-200
Plasmid#79602PurposeFUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sitesDepositorInsertd2GFP + miR-200 family targets
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 wt
Plasmid#80876Purposemammalian expression of ALK5 WTDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only