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pcDNA4/TO-BcRF1-2xStrep

Plasmid

#138436

Purpose

Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK K454R

Plasmid

#50501

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Use

Tags

HA

Expression

Mammalian

Mutation

K454R

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-LAP-KNL1-M3

Plasmid

#115896

Purpose

Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

Insert

KNL1-M3 (KNL1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Fragment of KNL1 containing three KNL1 repeat seq…

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PDE4D2cat-ICUE4

Plasmid

#181847

Purpose

ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)

Use

Tags

ECFP, PDE4D2(86-418), and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-GR-AKAR3

Plasmid

#173016

Purpose

Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Lyn-GR-AKAR3

Use

Tags

EGFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EPHA3_1-972 pcDNA3.1

Plasmid

#102739

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 MKK7b1(K149A)

Plasmid

#14674

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1(K149A) (Map2k7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Lys-149Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-YA/VD-GFP

Plasmid

#60703

Purpose

mammalian expression of rat Cx43 YA/VD mutant fused to GFP

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-3XHA-Zmat3

Plasmid

#167343

Purpose

Expresses HA-tagged Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

Zinc finger matrin-type 3 (Zmat3 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_LamB1

Plasmid

#181999

Purpose

CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf-LamB1

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/3xmyc_NLRP11-LRR

Plasmid

#131200

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

LRR of NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

contains only the leucine-rich repeats (LRRs), am…

Promoter

pCMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 K222R

Plasmid

#80887

Purpose

mammalian expression of ALK7 K222R

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-LmoCas6-p300

Plasmid

#126486

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-p300

Use

CRISPR

Tags

Flag and Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Actin/pcDNA3

Plasmid

#67776

Purpose

Actin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2v1-Fc(IgA1)

Plasmid

#145155

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 10 mut B-Myb

Plasmid

#25966

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (Mybl2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed threonines 267, 408, 497, 519, 522 and 52…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 1-590

Plasmid

#11624

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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