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pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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His-SUMO-hDDI2-D252N_pET11a

Plasmid

#169122

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Use

Tags

Expression

Bacterial

Mutation

D252N

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b T7pCONS TIR-2 sfGFP

Plasmid

#154468

Purpose

pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Use

Tags

His-thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a(+) 6xHis-3xFlag-IFIT5

Plasmid

#53561

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Use

Tags

3xFLAG, 6xHIS, and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
Mucin MUC1-5TR-pET15b

Plasmid

#174694

Purpose

Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

Depositor

Jean Gariepy

Article

Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

Insert

MUC1-TR

Use

Tags

hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(T21N)-RagC

Plasmid

#99644

Purpose

Bacterial expression of RagA(T21N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

T21N

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(D130N)-RagC

Plasmid

#99642

Purpose

Bacterial expression of RagA(D130N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D130N

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-AviTag-DogCatcher-MBP

Plasmid

#171928

Purpose

Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

AviTag-DogCatcher-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

DogCatcher added to N-terminus of MBP via a GSGES…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-ARH3-His-Sumo-HA

Plasmid

#111578

Purpose

Expresses ARH3 (amino acids 14-363) in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

ARH3 (ADPRS Human)

Use

Tags

6xHis-Sumo and HA

Expression

Bacterial

Mutation

deleted amino acids 1-13

Promoter

T7

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK104] pET28 SLC38A9N-His

Plasmid

#136142

Purpose

bacterial expression of SLC38A9 N term fragment (1-119)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(D181N)

Plasmid

#99643

Purpose

Bacterial expression of RagA-RagC(D181N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D181N

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-Ran(Q69L)

Plasmid

#171563

Purpose

Encodes human Ran protein

Depositor

Yan Zeng

Insert

Ran (RAN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_ALFA-tag-SNAP

Plasmid

#136628

Purpose

Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Use

Tags

8xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp2-Delta Card

Plasmid

#11810

Depositor

Guy Salvesen

Insert

Caspase-2 Delta Card (CASP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Delta Card

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT1

Plasmid

#53557

Purpose

bacterial expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
pET-EIN-AlkBH5(66-292)

Plasmid

#125732

Purpose

Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

Depositor

Vincenzo Venditti

Article

Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

Insert

AlkBH5(66-292) (ALKBH5 Human)

Use

Tags

6xHis and EIN

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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