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pENTR-L3-Cas9n-L2

Plasmid

#141039

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9n

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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dCas12Max-CBE

Plasmid

#195337

Purpose

vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-CBE

Use

Tags

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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dCas12Max-ABE

Plasmid

#195335

Purpose

vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-ABE

Use

Tags

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Shh

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Hsp68

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_U6-ωRNA*_ISDra2-TnpB-mNG

Plasmid

#212968

Purpose

AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

Depositor

Chunlong Xu

Article

Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

Inserts

ISDra2-TnpB-T2A-mNG,
ωRNA*

Use

AAV and CRISPR

Tags

NLS and NLS-FLAG

Expression

Mammalian

Mutation

Promoter

EF1a, T7 and U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

Plasmid

#112683

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-iCre and Nuclear localization signal

Expression

Mutation

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Expression

Mutation

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-nick sgRNA

Plasmid

#214096

Purpose

Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

KRAS G13A nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-tmpknot-epegRNA

Plasmid

#214091

Purpose

Lentiviral vector expressing epegRNA to induce GBA N370S mutation

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

GBA N370S epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170810

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SlugABE-NNG

Plasmid

#214356

Purpose

Expresses SlugABE-NNG

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG

Use

AAV

Tags

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_SpCas9_USP7 Exon 3 gRNA

Plasmid

#131257

Purpose

pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

humanised S. pyogenes Cas9 nuclease

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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UGPC

Plasmid

#138954

Purpose

Lentiviral vector for co-expressing Cre recombinase and sgRNA

Depositor

Nadya Dimitrova

Article

Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and PGK

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-tmpknot-epegRNA

Plasmid

#214092

Purpose

Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

EGFR L858R epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-T790M-tmpknot-epegRNA

Plasmid

#214093

Purpose

Lentiviral vector expressing epegRNA to induce EGFR T790M mutation

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

EGFR T790M epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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