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Plasmid#74049Purposeretroviral expression plasmid for human NFATc1/bCDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsno tagExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET22b-mEB3-eGFP-6xHis
Plasmid#130983PurposeBacterial expression of mouse EB3-GFP-6xHis for protein purification.DepositorInsertEB3-GFP-6xHis (Mapre3 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27A
Plasmid#89237PurposeExpression of GFP-tagged human Rab27A in mammalian cellsDepositorInserthuman Rab27A (RAB7A Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SB
Plasmid#177936PurposeExpresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFUW-tetO-GATA2
Plasmid#125028Purposedoxycycline-inducible expression of human GATA2 in mammalian cellsDepositorInsertGATA binding protein 2 (GATA2 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7_CC_PE7_IVT_Template
Plasmid#223022PurposeTemplate for in vitro transcription of PE7. For HSPC-related experiments.DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationPromoterT7Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV Δ18
Plasmid#169465PurposeEncodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))
Plasmid#218508Purposeintegrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertmNeongreen
UseTagsmodified ssrA degradation tagExpressionBacterialMutationdeletion of MCS containing Plac_lacZa, introducti…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-NG-403Q-Nterm-AAV
Plasmid#194651PurposeN-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in miceDepositorInsertABE8e-NG N-term
UseAAVTagsBPNLS and NpuN spilt inteinExpressionMutationPromoterTNNT2Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-NG-403Q-Cterm-AAV
Plasmid#194652PurposeC-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in miceDepositorInsertABE8e-NG C-term
UseAAVTagsBPNLS and NpuC split inteinExpressionMutationPromoterTNNT2Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP
Plasmid#155307PurposeRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFPDepositorInsertRfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
UseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.dCas9-KRAB.P2A.BSD
Plasmid#90332PurposeCRISPR interferenceDepositorInsertsdCas9
na
SFFV
P2A-BSD
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
cpVenus-Gγ2
Plasmid#69626PurposeThe heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).DepositorInsertcp173Venus-Ggamma2 (GNG2 Synthetic)
UseTagsciruclar permutated Venus (cp173V)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCAGImC_V5-CSNK1A1
Plasmid#92014PurposeExpresses V5-CSNK1A1, includes IRES-mCherryDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only