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Plasmid#185983PurposeMammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationP176L - mutation causing HSP (SPG58)PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_R169W-mCherry-FRB
Plasmid#185984PurposeMammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationR169W - mutation causing HSP (SPG58) and silent m…PromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQlinkG2-PTPIP51(236-470)
Plasmid#170534PurposeExpresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsGST tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(236-470)
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 neg ctrl
Plasmid#112030PurposeMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6KTN8
Plasmid#103076PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6KTN8(Cas9 coding gene from Actinomyces sp. oral taxon 180 str. F0310)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S and C699T)
Plasmid#52132Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470 and changed C478S and C6…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-470)
Plasmid#52125Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281 and 471-707PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7Jip1(282-470)
Plasmid#51703Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 1-281 and 471-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-GFP-CIBN
Plasmid#102252PurposeExpresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorInsertCIB1-VP64 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-mCherry
Plasmid#171573PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cellsDepositorInsertNb127D01-mCherry (CXCR2 Human)
UseTagsmCherryExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-tBid-pEGFP-C1
Plasmid#166736PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, tBidDepositorInserttBid (Bid Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIBN-rTetR
Plasmid#183919PurposeOptogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposureDepositorInsertCIBN-rTetR (CIB1 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 1)-myc
Plasmid#62845PurposeMammalian Expression of myc-tagged Opa1 isoform1DepositorInsertmmOPA1isoform1-myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianMutationPromotercmv-bactinAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMRX-IP-hWIPI1A-3×FLAG
Plasmid#215509PurposeExpresses FLAG tagged human WIPI1A.DepositorInsertWD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
UseRetroviralTags3×FLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only