Skip to main content
Addgene
Showing: 261 - 280 of 49191 results

  1. pShuttle

    Plasmid
    #67964
    Purpose
    Mobilizible plasmid with EGFP. OriT from the F plasmid.
    Depositor
    Joshua Leonard
    Insert
    OriT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67964-plasmid-map-sequence-id-121277

    Enlarge

  2. pX551-CMV-CjCas9

    Plasmid
    #107035
    Purpose
    AAV plasmid expressing CjCas9 under control of CMV promoter
    Depositor
    Alex Hewitt
    Insert
    CjCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107035-plasmid-map-sequence-id-208555

    Enlarge

  3. pMCSG53_Nsp15

    Plasmid
    #167265
    Purpose
    Bacterial expression of Nsp15
    Depositor
    Alexei Savchenko
    Insert
    Nsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    May 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167265-plasmid-map-sequence-id-331998

    Enlarge

  4. pX551-miniCMV-SaCas9

    Plasmid
    #107032
    Purpose
    AAV plasmid expressing SaCas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107032-plasmid-map-sequence-id-208547

    Enlarge

  5. pX551-miniCMV-CjCas9

    Plasmid
    #107033
    Purpose
    AAV plasmid expressing CjCas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    CjCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107033-plasmid-map-sequence-id-208551

    Enlarge

  7. pDEST26-hTLR3-Nluc

    Plasmid
    #197638
    Purpose
    Human CD283 protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR3 (TLR3 Human)
    Use
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197638-plasmid-map-sequence-id-392617

    Enlarge

  9. Top7 K39E K40E V48V K55E

    Plasmid
    #12464
    Purpose
    Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.
    Depositor
    David Baker
    Insert
    Top7 K39E K40E V46A K55E
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K39E K40E V46A K55E
    Promoter
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only
    12464-plasmid-map-sequence-id-235394

    Enlarge

  10. pAAVtri-CMV-eGFP

    Plasmid
    #230932
    Purpose
    Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.
    Depositor
    Qizhao Wang
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230932-plasmid-map-sequence-id-458356

    Enlarge

  12. TEF2pro_mCherry_YFP

    Plasmid
    #138623
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Rob Mitra
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    138623-plasmid-map-sequence-id-274912

    Enlarge

  13. TEF1pro_mCherry_YFP

    Plasmid
    #138624
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Rob Mitra
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    138624-plasmid-map-sequence-id-274931

    Enlarge

  14. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

    Enlarge

  15. pGBScrispri Sham

    Plasmid
    #223201
    Purpose
    Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223201-plasmid-map-sequence-id-451878

    Enlarge

  17. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

    Enlarge

  18. pMCSG53_N

    Plasmid
    #167255
    Purpose
    Bacterial expression of Nucleocapsid protein (residues 1-419)
    Depositor
    Alexei Savchenko
    Insert
    N protein (residues 1-419) (N SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167255-plasmid-map-sequence-id-331339

    Enlarge

  20. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

    Enlarge

Showing: 261 - 280 of 49191 results