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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

Plasmid

#186835

Purpose

Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 513/515/516 to Alanine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

Plasmid

#156443

Purpose

For transient expression of mouse BORIS (CTCFL) tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse BORIS (Ctcfl Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139485

Purpose

PCR template for Halo-Tag gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

HaloTag-loxP-P2A-dTKneo-pA-loxP

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139484

Purpose

PCR template for mEGFP gene knock-in donor vector

Depositor

Kazuhiro Aoki

Article

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

mEGFP-loxP-P2A-dTKneo-pA-loxP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

Plasmid

#128166

Purpose

Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-sfGFP-iCre-ERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1-IRES-mCherry

Plasmid

#232239

Purpose

Mammalian expression of REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro-GFP-BamHI-IRES-mCherry

Plasmid

#232247

Purpose

AcGFP1-BamHI-IRES-mCherry empty backbone

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

Plasmid

#223721

Purpose

Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

Depositor

Chiaki Murakami

Insert

DGKD (DGKD Human)

Use

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted sterile alpha motif domain (deleted amino…

Promoter

CAG and chicken β-actin promoter

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

Plasmid

#198069

Purpose

Mammalian expression of iRFP713(C15S/V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(C15S/V256C)

Use

Tags

P2A-mCherry

Expression

Mammalian

Mutation

C15S/V256C mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

Plasmid

#156436

Purpose

For transient expression of human PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5B (PDS5B Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

Plasmid

#156449

Purpose

For transient expression of human PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5A (PDS5A Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

Plasmid

#195101

Purpose

Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

CTCF (CTCF Human)

Use

Tags

AID, HA, and eGFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

Plasmid

#128167

Purpose

Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

Plasmid

#232246

Purpose

Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

delta73-82

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-RLIM-IRES-mCherry

Plasmid

#232243

Purpose

Mammalian expression of RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

RLIM-GFP-IRES-mCherry (RLIM Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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