Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 261 - 280 of 786 results

  4. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

    Enlarge

  5. pDONR P2R-P3-mKate2

    Plasmid
    #48345
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a stop codon at the 3' end of the c…
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    48345-plasmid-map-sequence-id-70403

    Enlarge

  8. pDONR P2R-P3-SV40 polyA signal

    Plasmid
    #48627
    Purpose
    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    SV40 early polyadenylation signal cassette
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    48627-plasmid-map-sequence-id-70632

    Enlarge

  9. pME-mTagBFP-CAAX

    Plasmid
    #75149
    Purpose
    Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mTagBFP-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    75149-plasmid-map-sequence-id-142103

    Enlarge

  10. pME-mVenus-CAAX

    Plasmid
    #75153
    Purpose
    Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mVenus-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    75153-plasmid-map-sequence-id-142107

    Enlarge

  11. pME-mKOFP2-CAAX

    Plasmid
    #75155
    Purpose
    Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    75155-plasmid-map-sequence-id-145998

    Enlarge

  12. p3E-mCherry-NS-MCS

    Plasmid
    #75179
    Purpose
    mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mCherry-NS-MCS
    Use
    Three prime entry vector for multisite gateway th…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    75179-plasmid-map-sequence-id-142186

    Enlarge

  13. p3E-EGFP-NS-MCS

    Plasmid
    #75180
    Purpose
    EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    EGFP-NS-MCS
    Use
    Three prime entry vector for multisite gateway th…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    75180-plasmid-map-sequence-id-153030

    Enlarge

  14. p3E-mTagBFP

    Plasmid
    #75175
    Purpose
    TagBFP (blue) fluorophore with stop codon for C-terminal fusions
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    TagBFP
    Use
    Three prime entry vector for multisite gateway th…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Availability
    Academic Institutions and Nonprofits only
    75175-plasmid-map-sequence-id-154499

    Enlarge

  15. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR; Gateway entry vector
    Tags
    Expression
    Mutation
    Added 3 stop codons to the original sequence; see…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113745-plasmid-map-sequence-id-260896

    Enlarge

Showing: 261 - 280 of 786 results