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Plasmid#131757PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expressionDepositorInsertTRE#2 Sp/xCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA
Plasmid#131756PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expressionDepositorInsertTRE#1 Sp/xCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA
Plasmid#131755PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expressionDepositorInsertTRE#1 SaCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA
Plasmid#131758PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expressionDepositorInsertTRE#2 SaCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold
Plasmid#121842PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expressionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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S3_001_026_096
Plasmid#134402PurposepYTK096-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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S3_001_026_MV2
Plasmid#134422PurposepYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_002_026_096
Plasmid#134403PurposepYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsNone and noneExpressionYeastMutationNone and nonePromoterpGPM1 and pRPL18BAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_004_026_MV1
Plasmid#134416PurposepYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_007_026_MV1
Plasmid#134419PurposepYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_008_026_MV1
Plasmid#134420PurposepYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pRPL18BAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_009_026_MV1
Plasmid#134421PurposepYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_007_026_MV2
Plasmid#134424PurposepYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_007_026_096
Plasmid#134410PurposepYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_008_026_096
Plasmid#134411PurposepYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pRPL18BAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
S3_009_026_096
Plasmid#134412PurposepYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pREV1Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_004_026_096
Plasmid#134407PurposepYTK096-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGPM1 and pTDH3Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S3_002_026_MV1
Plasmid#134414PurposepYTKMV1-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pRPL18BAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only