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Showing: 261 - 280 of 545 results

  1. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176577
    Purpose
    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Hexahistidine tag and mVenus
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176577-plasmid-map-sequence-id-348269

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  2. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid
    #174635
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174635-plasmid-map-sequence-id-346993

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  6. pRDAX-VALENTIA-028 EB3-mVenus//BsaI

    Plasmid
    #130209
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    EB3-mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    130209-plasmid-map-sequence-id-273537

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  7. pRDAX-VALENTIA-026 Lyn-mVenus//BsaI

    Plasmid
    #130207
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    Lyn-mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    130207-plasmid-map-sequence-id-273534

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  8. pRDAX-VALENTIA-025 Lamp1-mVenus//BsaI

    Plasmid
    #130206
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    Lamp1-mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    130206-plasmid-map-sequence-id-273533

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  10. pRDAX-VALENTIA-014 EB3-mVenus//BsaI

    Plasmid
    #130195
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    EB3-mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    130195-plasmid-map-sequence-id-273425

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  12. pRDAX-VALENTIA-004 Lyn-mVenus//BsaI

    Plasmid
    #130185
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    Lyn-mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    130185-plasmid-map-sequence-id-272490

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  13. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  18. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid
    #193721
    Purpose
    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193721-plasmid-map-sequence-id-383728

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  19. pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

    Plasmid
    #174641
    Purpose
    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(micro)-Venus
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    174641-plasmid-map-sequence-id-355829

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  20. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

    Plasmid
    #174637
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174637-plasmid-map-sequence-id-346992

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Showing: 261 - 280 of 545 results