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Plasmid#160866PurposePJ23110-RBS-CAT-taa-mRuby2-tL3S2P24_KanR_p15ADepositorInsertChloramphenicol Acetyltransferase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23110_BBa_B0029Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-postASAP-p2a-ChrimsonR
Plasmid#178794PurposeExpresses voltage sensor targeted to dendrites and spines along with ChrimsonRDepositorInsertFingR.PSD95, ASAP2f and ChrimsonR
UseTagsmRuby2ExpressionMammalianMutationmutations in amino acids L146G, S147T N149R, S150…PromoterCAGAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Ruby2-NS
Plasmid#166569PurposeMultisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJADepositorInsertRuby2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW842_pCAG-pExpr-FP-DECODER
Plasmid#87551PurposeExpresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinasesDepositorInsertmtagBFP, EGFP, iRFP720, mRuby2
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.244
Plasmid#73756PurposeNabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.244
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.242
Plasmid#73754PurposeNabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.242
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.30
Plasmid#73798PurposeNabi2.30 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.30
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.104
Plasmid#73799PurposeNabi2.104 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.104
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.125
Plasmid#73800PurposeNabi2.125 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.125
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.216
Plasmid#73801PurposeNabi2.216 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.216
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.226
Plasmid#73802PurposeNabi2.226 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.226
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.213
Plasmid#73757PurposeNabi2.213 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.213
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKL2-160
Plasmid#219414PurposeEstradiol-inducible expression of 13-6 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS2389-Tier1-PhCMV-p2a_mRuby
Plasmid#169526PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).DepositorInsertPCMV-driven P2A-fused enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationFour Arg to Ala mutations in zinc finger backbonePromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorInsertGFP11-RhoA (RHOA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Pansio
Plasmid#214445PurposeExpresses eSpCas9 and gRNA targeting Pansio-1 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Olônne
Plasmid#214447PurposeExpresses eSpCas9 and gRNA targeting Olônne-18 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only