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BmTyr-Flag
Plasmid#220779Purposenon-targeted BmTyr throughout the cytoplasmDepositorInsertBacillus megaterium Tyrosinase
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP2-Flag
Plasmid#210344PurposeExpresses SARS-CoV-2 NSP2 in mammalian cellsDepositorInsertSARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ALX4-Flag
Plasmid#215029PurposeMammalian expression of human ALX4DepositorInsertALX homeobox 4 (ALX4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG NC
Plasmid#214675PurposeNegative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tagsDepositorInsertFLAG
UseTagsMCP and V5ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-KIF5AR280H
Plasmid#166957PurposeExpresses FLAG-tagged KIF5A R280H protein in pcDNA3.1DepositorInsertKIF5A-R280H (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A-R280HPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD4L6
Plasmid#153157PurposeExpression of Flag tagged human FOXD4L6 in mammalian cellsDepositorInserthuman FOXD4L6 (FOXD4L6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE99Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD4L1
Plasmid#153154PurposeExpression of Flag tagged human FOXD4L1in mammalian cellsDepositorInserthuman FOXD4L1 (FOXD4L1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE96Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD4L3
Plasmid#153155PurposeExpression of Flag tagged human FOXD4L3 in mammalian cellsDepositorInserthuman FOXD4L3 (FOXD4L3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE97Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-hCCDC11
Plasmid#122993PurposeExpresses Flag-tagged human CCDC11 in mammalian cellsDepositorInserthuman CCDC11 (CFAP53 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125234PurposeExpresses SPARK oβ2AR transmembrane component in mammalian cellsDepositorInsertgLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187611PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationPromoterEF1AAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187612PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain (R163A) with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationArg163AlaPromoterEF1AAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_3XFlag
Plasmid#182494PurposeEmpty plasmid containing Flag tag for C-terminal taggingDepositorInsert3xFlag with 5 Glycolic spacer
UseTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIP
Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertring finger protein 31 (RNF31 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_3Flag
Plasmid#182496PurposeContains cDNA of wt METDepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 (EZH2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationExon 14 deletedPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only
