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Plasmid#110594PurposeBTK Type 4 terminator for golden gate assemblyDepositorInsertT7 terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmRE-Tn5-142
Plasmid#118531PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK224
Plasmid#110590PurposeBTK Type 3 coding sequence for golden gate assemblyDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK110
Plasmid#110576PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP6 Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK112
Plasmid#110577PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP12b Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK113
Plasmid#110578PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP32 Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRTagsExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM4941 (pCV0053)
Plasmid#120083PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM4937 (pCV0049)
Plasmid#120082PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK615
Plasmid#110609PurposeBTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9DepositorInsertsgRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM4951 (pCV0063)
Plasmid#120084PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
UseTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH18
Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNAAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK614
Plasmid#110608PurposeBTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK301
Plasmid#110593PurposeBTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)DepositorInsertBba_B0015 Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMF440
Plasmid#62550PurposeBroad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUG-natNT2
Plasmid#110922Purposeexpression of nat gene conferring resistance to nourseothricin for gene deletion in yeastDepositorInsertnat
UseTagsExpressionBacterial and YeastMutationPromoterAgTEF1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5057
Plasmid#120085PurposeBroad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.DepositorInsertRSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJN105-Hygro-GFP
Plasmid#41883DepositorInsertPtac::GFP-mut-2
UseBacterial broad host range vectorTagsExpressionBacterialMutationGFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…PromoterPtacAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pstb-LAFR5
Plasmid#86169PurposepLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSMb21651 promoterAvailable sinceFeb. 6, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits