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Showing: 261 - 280 of 411 results

  5. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Article
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Availability
    Industry, Academic Institutions, and Nonprofits
    85168-plasmid-map-sequence-id-372970

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  6. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  13. pAH18

    Plasmid
    #154397
    Purpose
    Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Availability
    Academic Institutions and Nonprofits only
    154397-plasmid-map-sequence-id-308811

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  14. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

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  15. pMF440

    Plasmid
    #62550
    Purpose
    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
    Depositor
    Michael Franklin
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Availability
    Academic Institutions and Nonprofits only
    62550-plasmid-map-sequence-id-397042

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  17. pAM5057

    Plasmid
    #120085
    Purpose
    Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.
    Depositor
    Susan Golden
    Article
    Ma et al Appl Environ Microbiol. 2014 Nov;80(21):
    Insert
    RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120085-plasmid-map-sequence-id-234368

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  18. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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Showing: 261 - 280 of 411 results