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Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
7iBi89 (rat fibronectin)
Plasmid#14065DepositorInsertRat Fibronectin 7iBi89 (Fn1 Rat)
UseTagsExpressionMammalianMutationInsert was cut out of pGEM3 with SalI and SacI an…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
MXS_MCS1
Plasmid#62395PurposeMXS_chaining vector with MCS1DepositorInsertEcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) HIS-tag pET
Plasmid#8492DepositorInsertHoxA9 (Hoxa9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationBamHI/BalI fusion into amino acid #2 of HOXA9; ma…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG-Nde-Acc
Plasmid#33150DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
UseTagsLacZExpressionYeastMutationAccI site in intron was cut/filled/ligated to cha…PromoterAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
9X NFAT-apha-MHC-Luc
Plasmid#51941Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3DepositorInserts9x NFAT binding site
alpha-MHC
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFU-GW-sMYH6-mNeon-TNNT2-mScarlet
Plasmid#170712Purposedual fluorescent reporter for MYH6 (short) and TNNT2 promotersDepositorInsertsmNeonGreen
mScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterTNNT2 and aMHCAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
YF-Cdc42(CA)
Plasmid#20152DepositorInsertYFP-FKBP-Cdc42(CA) (CDC42 Human)
UseTagsExpressionMammalianMutationCdc42H was inserted into the multiple cloning sit…PromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A/T1462A)
Plasmid#14131DepositorInsertTSC2 S939A/T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationS939A/T1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2
Plasmid#109289PurposeExpresses high levels of protein in mycobacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationpSUM-kan-MCS1-gfp was modified to reorient the Ba…PromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorInsertTSC2 T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationT1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (S939A)
Plasmid#14132DepositorInsertTSC2 S939A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationS939A.PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-GAAZFP-FokI-RR
Plasmid#18754DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only