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N-4-BE

Plasmid

#135019

Purpose

C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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PILAR

Plasmid

#22851

Depositor

Jose Conejo-Garcia

Article

Huarte et al Blood. 2008 Aug 15. 112(4):1259-68.

Insert

PILAR (CLEC2A Human)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only
pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70

Plasmid

#46124

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCfB2223

Plasmid

#67544

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLei-wt-sfGFP

Plasmid

#191113

Purpose

An E. coli sfGFP wild type control

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-MfnG-TEV-His

Plasmid

#191114

Purpose

Expresses MfnG-TEV-His in E. coli

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMK484 (2mAID-Hygro)

Plasmid

#214396

Purpose

2mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

2mAID-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK485 (3mAID-Hygro)

Plasmid

#214397

Purpose

3mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

3mAID-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK454 (dTAG-BSD)

Plasmid

#214384

Purpose

dTAG for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

dTAG-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.1

Plasmid

#208300

Purpose

Expresses ABE-RA2.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, D108G, K110R, H122R, M126I, N127K

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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FB430

Plasmid

#203621

Purpose

TU for the expression of phleomycin resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PpcbC:ble:TamdS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB431

Plasmid

#203622

Purpose

TU for the expression of terbinafine resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PgpdA:ergA:TamdS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBM10a

Plasmid

#179688

Purpose

Target plasmid for context profiling

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

ACA target

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-A_GFP_TCYC1

Plasmid

#177162

Purpose

Expresses yeGFP under the control of a synthetic promoter (PGAL7-A)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-A)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-B_GFP_TCYC1

Plasmid

#177163

Purpose

Expresses yeGFP under the control of a synthetic promoter (PGAL7-B)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-B)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-C_RFP_TCYC1

Plasmid

#177164

Purpose

Expresses yeRFP under the control of a synthetic promoter (PGAL7-C)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-C)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-D_RFP_TCYC1

Plasmid

#177165

Purpose

Expresses yeRFP under the control of a synthetic promoter (PGAL7-D)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-D)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMV23

Plasmid

#128887

Purpose

Position 4 module- STOP (stop codon)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

STOP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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