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Plasmid#68763PurposeZF9-TF-V2 with N-FLAG and C-HISDepositorInsertZF9-TF-V2
UseTagsHIS, NLS, and flagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-247
Plasmid#68761PurposeZF9-TF-V2 with mCherry markerDepositorInsertsZF9-TF-V2
mCherry
UseTagsNLS and flagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-345
Plasmid#68785PurposeR->A Sensor pair 1 with mCherry markerDepositorInsertsC-Terminal Sensor 1 V2 R-->A mutant
N-Terminal Sensor 1 V2 R-->A mutant
mCherry
UseTagsNLS, flag, and nlsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSgk1_v2 Flag
Plasmid#86040Purposefull length human Sgk1_i2 with Flag tagDepositorInsertAlternate transcript v2 of human Sgk1 (SGK1 Human)
UseTagsFLAGExpressionMammalianMutationv2PromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2
Plasmid#214814PurposeA single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATMDepositorInsertSt1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
LcV2-Hygro
Plasmid#91977PurposeLentiCRISPR v2 with Hygro cloned in place of PuroDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB117 - pL2_pSB90_fLUC-I_V2
Plasmid#123192Purposebinary plant vector for transient TYLCV V2 and fLUC (with intron) expressionDepositorInsert2x35S::V2::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_4
Plasmid#192688PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_1
Plasmid#192685PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_2
Plasmid#192686PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_3
Plasmid#192687PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSynapsin-PE2-Cterminal-lenti
Plasmid#135956PurposeNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) C-terminal
UseLentiviralTagsExpressionMammalianMutationMMLV RT(D200N, T306K, W313F, T330P, L603W)PromoterhSynapsinAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti
Plasmid#135955PurposeNeuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) N-terminal
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)
Plasmid#133793PurposeU6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017DepositorInsertCjeCas9 sgRNA entry vector
UseTagsExpressionMammalianMutationV2-TGAA linker sgRNA architecture from Kim et al.…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPD301 ZF43x16-Compact_EYFP
Plasmid#138911PurposeEYFP reporter vector for the ZF43x16-Compact promoterDepositorInsertZF43x16-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x16-CompactAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD299 ZF43x14-Compact_EYFP
Plasmid#138909PurposeEYFP reporter vector for the ZF43x14-Compact promoterDepositorInsertZF43x14-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x14-CompactAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD294 ZF43x10-Compact_EYFP
Plasmid#138905PurposeEYFP reporter vector for the ZF43x10-Compact promoterDepositorInsertZF43x10-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x10-CompactAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD300 ZF43x15-Compact_EYFP
Plasmid#138910PurposeEYFP reporter vector for the ZF43x15-Compact promoterDepositorInsertZF43x15-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x15-CompactAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only