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Plasmid#128882PurposePosition 3 module- sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyTagsExpressionBacterialMutationF11V, T13P, N39D, A179APromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor lambda version
Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM005
Plasmid#170009PurposeEncodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PvirKAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM002
Plasmid#170006PurposeEncodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PpmrDAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB4A5 JQ929581
Plasmid#38168DepositorInsertsmKate2
Superfolder GFP
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS52
Plasmid#104334PurposeExpresses sfGFP from the OmpR regulated PompF promoter.DepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterPompFAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVHed02
Plasmid#82352PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB4A5 JQ929582
Plasmid#38169DepositorInsertsmKate2
Superfolder GFP
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Phi80 JQ929581
Plasmid#38170DepositorInsertsmKate2
Superfolder GFP
UseSynthetic Biology; Integration vectorTagsExpressionMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-196a.M3
Plasmid#220467PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7
Plasmid#220464PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7.M4
Plasmid#220465PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-196a
Plasmid#220466PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDA313
Plasmid#195214PurposeExpression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.DepositorInsertsfGFP with tetO and a hairpin
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0296
Plasmid#162305PurposeLevel 0 CDS part for MoClo assembly, AATG-TTCGDepositorInsertCDS, superfolder GFP, no stop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVHed07
Plasmid#82350PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Phi80 JQ929582
Plasmid#38200DepositorInsertsmKate2
Superfolder GFP
UseSynthetic Biology; Integration vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM003
Plasmid#170007PurposeEncodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PmgtCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only