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Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
UseTagsExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
UseTagsExpressionMammalianMutationC289TPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum_C207G/T208C
Plasmid#177831PurposeSite-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).DepositorInsertmPlum_C207G/T208C
UseTagsExpressionMammalianMutationC207G + T208CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES795
Plasmid#87943PurposeConstitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMars1CyeGFP
Plasmid#75222PurposeMammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusionDepositorInsertMars1Cy-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pP2AeGFP-Mars1Cy
Plasmid#75223PurposeMammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFPDepositorInsertEGFP-p2a-Mars1Cy
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARB367
Plasmid#124050PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN2981
Plasmid#127246PurposeAsymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFPDepositorInsertpPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN1579
Plasmid#127201PurposeProgesterone inducible expression of KeyC-BFPDepositorInsertpPAB1-Z3PM-tTDH1-pZ3-key_C-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN1935
Plasmid#127203PurposeConstitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchCDepositorInsertpTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN1572
Plasmid#127202PurposeDual inducible expression of Venus-degronSwitchA and KeyA-BFPDepositorInsertpGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN1578
Plasmid#127200PurposeProgesterone inducible expression of KeyA-BFPDepositorInsertpPAB1-Z3PM-tTDH1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAN1735
Plasmid#127204PurposeDual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLSDepositorInsertpGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Lrrc40-trunc-IRES-BFP
Plasmid#117268PurposeRetroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFPDepositorInsertLrrc40 (Lrrc40 Mouse)
UseRetroviralTagsExpressionMammalianMutationThe insert is truncated - deletion of bp 334-407 …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOIMars1CyN
Plasmid#75221PurposeMammalian cell surface expression of fluorogen activating protein Mars1CyDepositorInsertss-HA-Mars1Cy-CD80(237-306)
UseTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1
Plasmid#176839PurposeTo recruit SAM complex to human CYP3A4 proximal enhancer.DepositorInsertHuman CYP3A4 proximal enhancer sgRNA for activation
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-D387A
Plasmid#59778PurposeExpresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by lightDepositorInsertOptoFGFR1-D387A (FGFR1 Mustard Weed, Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only