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pcF-3

Plasmid

#131024

Purpose

Single spacer array with spacer #3 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcF-3/2/1

Plasmid

#131031

Purpose

Three-spacer array 6 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-2/3/1

Plasmid

#131030

Purpose

Three-spacer array 5 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-3/1/2

Plasmid

#131029

Purpose

Three-spacer array 4 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-2/1/3

Plasmid

#131028

Purpose

Three-spacer array 3 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmAGO1

Plasmid

#22768

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1

Plasmid

#22420

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPathBrick

Plasmid

#65006

Purpose

E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive native promoters

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::UMSBP::sNluc2_ORF1

Plasmid

#186765

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAPDH::sNluc1

Plasmid

#187221

Purpose

A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Overexpression

Tags

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::ENO::sNluc2

Plasmid

#186770

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc0

Plasmid

#186771

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::hNluc

Plasmid

#186772

Purpose

An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::hNluc

Plasmid

#186774

Purpose

An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc1

Plasmid

#186754

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::ENO::sNluc1

Plasmid

#186755

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL10::sNluc1

Plasmid

#186756

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc1

Plasmid

#186757

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge