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Plasmid#203657PurposeYeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsGFPMUT3ExpressionYeastMutationPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry
Plasmid#203652PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.DepositorArticleInsertPfV
UseLentiviralTagsSapphire-IRES-H2B-mCherryExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-UBF
Plasmid#26672DepositorInsertUBF1 (UBTF Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNUT3
Plasmid#175744PurposeSingle-component photo-inducible (CRY2-based) nucleolus-targeting tool 3DepositorInsertmCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT2
Plasmid#175745PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 2DepositorInsertmCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS4A1 RFP (805)
Plasmid#62009Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertMS4A1
UseTagsmRFPExpressionMammalianMutationPromoterpgkAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Fibrillarin
Plasmid#26673DepositorInsertFibrillarin (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45564PurposeExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequenceDepositorInsertsd14 eGFP donor
I-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationdeleted n' 15 amino acidsPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
NKP64 GFP (1007)
Plasmid#62020Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertSLC38A10 (SLC38A10 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-GAAZFP-FokI-RR
Plasmid#18754DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-TTGZFP-FokI-DD
Plasmid#18755DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
NKP33 GFP (1032)
Plasmid#62022Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertC9orf46 (PLGRKT Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB-nHyPer2 (NLS:HyPer2:NLS)
Plasmid#84730PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to NucleiDepositorInsertHyPer2 targeted to nucleus
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEndo-I-OnuI-TSM
Plasmid#207954PurposeExpresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.DepositorInsertThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
UseSynthetic BiologyTagsExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in fu…PromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NKP38 GFP (1009)
Plasmid#62021Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertAYTL1 (LPCAT2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-ICUE3-NLS
Plasmid#138255PurposeFRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertFKBP-ICUE3-NLS
UseTagsFKBP, Nuclear localization signal (NLS), cpVenus[…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EXO1a
Plasmid#111629PurposeExpresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cellsDepositorInsertExonuclease 1a (EXO1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374delNLS2
Plasmid#62532PurposeN-terminal aa1-269 and nuclear localization signal 2 of Drosha are deletedDepositorInserttruncated human Drosha mutant (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-269 and nuclear localizatio…PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein G51D (SNCA Human)
UseTagsExpressionBacterialMutationG51DPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only