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pCAG_Xph20-mEos3.2-CCR5TC

Plasmid

#135532

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Thomas Pollard, Julien Berro

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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RHPMP139 (pLifeAct mEOS3.1)

Plasmid

#88839

Purpose

Localization by Super-Resolution microscopy of lifeAct fused to mOrange2

Depositor

Ricardo Henriques

Insert

LifeAct

Use

Tags

mEOS3.1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE mEos3.2-Camk2a KI

Plasmid

#131493

Purpose

Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and mEos3.2 donor (Camk2a, Camk2a Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Integrin-Beta1-N-18

Plasmid

#57390

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta1 (ITGB1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Integrin-Alpha2-N-18

Plasmid

#57389

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Alpha2 (ITGA2 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pJB042 (FtsZ-mEos2)

Plasmid

#49764

Purpose

Inducible expression of FtsZ-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

Insert

FtsZ-mEos2

Use

Tags

mEos2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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mEos2-Integrin-Beta3-N-18

Plasmid

#57391

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta3

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pirespuro2-CD86-mEos2

Plasmid

#98284

Purpose

mammalian expression of CD86-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CD86 (CD86 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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VSVG-mEOS2-2xUVR8

Plasmid

#49803

Purpose

expresses VSVG fused to UVR8 for light-triggered trafficking

Depositor

Matthew Kennedy

Article

Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

Insert

VSVG-mEOS-2xUVR8

Use

Tags

HA, MYC, and mEOS2

Expression

Mammalian

Mutation

VSVG contains ts045 mutations

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His-mEos3.2

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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HA-mEOS3.2-TRF2 HR Donor

Plasmid

#86845

Purpose

HR Donor for N-terminal HA-mEOS3.2-TRF2 fusion at endogenous TRF2 locus in human cells.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

HA-mEOS3.2-TRF2 at endogenous locus (TERF2 Human)

Use

Tags

HA-mEOS3.2

Expression

Mammalian

Mutation

Promoter

Endogenous TRF2 promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-V-Integrin-25

Plasmid

#57345

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Catherine Galbraith

Article

Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Use

Tags

pr-mEosFP

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos3.2-A69T

Plasmid

#98571

Purpose

photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos3.2-A69T

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-Beta-Catenin-20

Plasmid

#57350

Purpose

Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Beta-Catenin (Ctnnb1 Mouse)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Alpha-Actinin-19

Plasmid

#57346

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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