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Plasmid#199496PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2AD273K
Plasmid#202727Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2- PIP4K2BD278K
Plasmid#202729Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2- PIP4K2CD280K
Plasmid#202731Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2C (D280K) (PIP4K2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC_hU6-shRKIP_BFP
Plasmid#199216PurposeExpression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.DepositorInsertshBACH1 (BACH1 Synthetic)
UseRNAiTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2
Plasmid#167897PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2357_Tier3(PB)-TagBFP_BlastR
Plasmid#169656PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX208-nfBFP
Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralTagsExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.mTagBFP2.miR30n
Plasmid#169281PurposeConstitutive overexpression of miRNA or shRNA with mTagBFP2 as reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-ECFP
Plasmid#118854Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-ECFP
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-mTFP1
Plasmid#118855Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-mTFP1
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe2034 HC_Kan_mTagBFP2_p9
Plasmid#100689Purposepart designed to occupy position 9 of EMMA. Functional category: Fluorescent markerDepositorInsertmTAGBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Myotilin-C-14
Plasmid#55316PurposeLocalization: Cytoskeleton, Excitation: 399, Emission: 456DepositorInsertMyotilin (MYOT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKAM-BFP-Puro
Plasmid#101864PurposeLentiviral empty backbone, dual BFP and puro markersDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-PCNA-19-SV40NLS-4
Plasmid#55320PurposeLocalization: Nucleus, Excitation: 399, Emission: 456DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and mTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
lenti-mito-mTagBFP2
Plasmid#216130PurposeLentiviral vector for expressing mTagBFP2 localizing to mitochondria.DepositorInsertEF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2
Plasmid#191566PurposeExpresses an shRNA and mTagBFP2DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-BFP
Plasmid#78547PurposeCas9 protein with BFPDepositorInsertT2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
NES-mTagBFP2-P4Mx1
Plasmid#108142PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only