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Plasmid#110248PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRG9MTD1 (TRMT10C Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-3xFLAG/HA/NBL10
Plasmid#110568PurposeCre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tagDepositorInsert3xFLAG/HA-tagged anti-GFP VHH
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2497
Plasmid#110942PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviralTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV400
Plasmid#110721PurposeLentiviral system for reduced ectopic expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RAD51_Rad51
Plasmid#110031PurposeProtein expression and purification of RAD51_Rad51DepositorInsertRAD51_Rad51 (RAD51 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionBacterialMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK121
Plasmid#110581PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertPA3 Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D
Plasmid#110300PurposeExpresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.wt
Plasmid#110346PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK599
Plasmid#110600PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-puro-tetON-shOGDH 1714
Plasmid#110941PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseRNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
KALRN (KALRNA-c001)
Plasmid#110256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKALRN (KALRN Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB364
Plasmid#110275PurposeN-terminal 10-His DsbC TEV-peptide fusionDepositorTypeEmpty backboneUseTags10-His, TEV cleavage peptide, and mature DsbC (i.…ExpressionBacterialMutationPromoterT7Available sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shLuc
Plasmid#110409PurposeshLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.DepositorInsertLuciferase
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN1
Plasmid#110634PurposeRetroviral expression of Flag-SFXN1 in mammalian cellsDepositorInsertSFXN1 (SFXN1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q145-Myc-B
Plasmid#110489PurposeLentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationpartial long 3'UTR wild type huntingtin exon…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR3C1_Hormone-recep
Plasmid#110018PurposeProtein expression and purification of NR3C1_Hormone-recepDepositorInsertNR3C1_Hormone-recep (NR3C1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-eGFP
Plasmid#110693PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFPDepositorInsertCPSF6-eGFP (CPSF6 )
UseLentiviralTagsEGFPExpressionMutationTruncation of CPSF6 isoform 2 at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only