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Plasmid#112286PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Serine 94 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CBh-dCas9-hUtx-IRES-Hyg
Plasmid#122374PurposeExpresses dCas9-hUtx-IRES-Hyg in mammalian cellsDepositorInsertCBh-dCas9-hUtx-IRES-Hyg-pA
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF (2XPDZ-mCherry-Larg (DH)
Plasmid#80407PurposeRecruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorInsert2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
UseTagsmCherryExpressionMammalianMutationDH domain only (aa 766-997)PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-mCherry-Syk
Plasmid#50045PurposeExpression of mCherry-Syk in mammalian cellsDepositorInsertSpleen tyrosine kinase (Syk) (Syk Mouse)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV.PGK.mFoxa2
Plasmid#33014DepositorInsertForkhead box A2 (Foxa2 Mouse)
UseLentiviralTagsExpressionMammalianMutationH10LPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc
Plasmid#136438PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 FcDepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524)PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
NUPL2_ZNF_pGEX
Plasmid#135135PurposeRecombinant expression of NUPL2 with dual-affinity tagDepositorInsertNUPL2 (NUPL2 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (Merlin)
Plasmid#136452PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CBh-dCas9-hEzh2-IRES-Hyg
Plasmid#122375PurposeExpresses dCas9-hEzh2-IRES-Hyg in mammalian cellsDepositorInsertCBh-dCas9-hEzh2-IRES-Hyg-pA
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dnmt3b-R
Plasmid#122333PurposeExpresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dnmt3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZapLck biosensor
Plasmid#131584PurposeLck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRETDepositorInsertZapLck biosensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-Gsk3b(new)
Plasmid#122341PurposeExpresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cellsDepositorInsertsgRNA for mouse Gsk3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PUM1_PUM_pGEX
Plasmid#135129PurposeRecombinant expression of PUM1 with dual-affinity tagDepositorInsertPUM1 (PUM1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (TB40/E)
Plasmid#136445PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-717). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (TB40/E)
Plasmid#136453PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tagDepositorInsertenvelope glycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - BirA*
Plasmid#131132PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[1-20]-N-int
Plasmid#199201PurposeGateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expressionDepositorInsertsplit-intein-Gal4[1-20]-N-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNRPA_Full-length_pGEX
Plasmid#135134PurposeRecombinant expression of SNRPA with dual-affinity tagDepositorInsertSNRPA (SNRPA Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
plexm-human LRRC33
Plasmid#111600PurposeExpress human LRRC33 in 393T cellsDepositorInsertLRRC33 (NRROS Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBM23_2xRRM_pGEX
Plasmid#135118PurposeRecombinant expression of RBM23 with dual-affinity tagDepositorInsertRBM23 (RBM23 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAX-EGFP-FBP17
Plasmid#178365PurposeExpresses FBP17 c-terminal labeled with EGFP in mammalian cellsDepositorInsertFormin Binding Protein 17 (FNBP1 Human)
UseTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (TB40/E)
Plasmid#136451PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dnmt3b-L
Plasmid#122334PurposeExpresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dnmt3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TAF15_RRM-ZNF_pGEX
Plasmid#135111PurposeRecombinant expression of TAF15 with dual-affinity tagDepositorInsertTAF15 (TAF15 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC4_pLib
Plasmid#173681PurposeArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC4 (ARPC4 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
RBFOX3_RRM_pGEX
Plasmid#135131PurposeRecombinant expression of RBFOX3 with dual-affinity tagDepositorInsertRBFOX3 (RBFOX3 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SRSF11_RRM_pGEX
Plasmid#135121PurposeRecombinant expression of SRSF11 with dual-affinity tagDepositorInsertSRSF11 (SRSF11 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceOct. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ArpC5_pLib
Plasmid#173682PurposeArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC5 (ARPC5 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
RBM45_Full-length_pGEX
Plasmid#135127PurposeRecombinant expression of RBM45 with dual-affinity tagDepositorInsertRBM45 (RBM45 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
NOVA1_Full-length_pGEX
Plasmid#135147PurposeRecombinant expression of NOVA1 with dual-affinity tagDepositorInsertNOVA1 (NOVA1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)
Plasmid#159658PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)
Plasmid#159657PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Eif4a1-R
Plasmid#122345PurposeExpresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Eif4a1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
RBM4_2xRRM-ZNF_pGEX
Plasmid#135154PurposeRecombinant expression of RBM4 with dual-affinity tagDepositorInsertRBM4 (RBM4 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCBP4_3xKH_pGEX
Plasmid#135156PurposeRecombinant expression of PCBP4 with dual-affinity tagDepositorInsertPCBP4 (PCBP4 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTV-mHdac1-tdiRFP670-PEST2
Plasmid#122351PurposeTargeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.DepositorInsert2A-tdiRFP-PEST with homology arms for Hdac1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-Y206S
Plasmid#136435PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY206S (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-V242K
Plasmid#136436PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationV242K (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-Met-Eif4a1-tdiRFP670-PEST
Plasmid#122371PurposeTargeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.DepositorInsert2A-tdiRFP-PEST-2A with homology arms for Eif4a1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Eif4a1-L
Plasmid#122346PurposeExpresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Eif4a1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
UNK_6xZNF_pGEX
Plasmid#215703PurposeRecombinant expression of UNK with dual-affinity tagDepositorInsertUNK (UNK Human)
UseTagsGST and SBPExpressionBacterialMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Msi1_2xRRM_pGEX
Plasmid#215704PurposeRecombinant expression of mouse Msi1 with dual-affinity tagDepositorInsertMusashi-1 (Msi1 Mouse)
UseTagsGST and SBPExpressionBacterialMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-wt-HaloTag
Plasmid#201551PurposeExpresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1 (partial)
UseTagsHaloTagExpressionInsectMutationCodon-optimized for Drosophila expressionPromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-wt
Plasmid#201553PurposeExpresses wild-type Octopus synaptotagmin-1 C2A domain in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationCodon-optimized for E. coliPromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pCAGGS
Plasmid#206052Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pcDNA3
Plasmid#206051Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS pcDNA3
Plasmid#206057Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only