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Showing: 651 - 700 of 2220 results

  2. pJK274

    Plasmid
    #71564
    Purpose
    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast
    Depositor
    T. J. Kappock
    Article
    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007
    Insert
    delta12 fatty acid desaturase (form 3)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    GAL1 promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71564-plasmid-map-sequence-id-129501

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  4. pCVD518

    Plasmid
    #66158
    Purpose
    Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe
    Depositor
    James Kaper
    Article
    Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.
    Insert
    tdh1
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pCVD531

    Plasmid
    #66160
    Purpose
    A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned into the HindIII site of pBR322.
    Depositor
    James Kaper
    Article
    Nishibuchi et al FEMS Microbiol Lett. 1990 Feb;55(3):251-
    Insert
    tdh-like
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pCVD437

    Plasmid
    #63758
    Purpose
    7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pCVD434

    Plasmid
    #63757
    Purpose
    EPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae probe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pACCS3-1

    Plasmid
    #78559
    Purpose
    4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.
    Depositor
    James Kaper
    Article
    Giron et al Vaccine. 1995 Jul;13(10):939-46.
    Insert
    cs3 operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tet
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. pJK511

    Plasmid
    #71591
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)
    Depositor
    T. J. Kappock
    Article
    Murphy et al Acta Crystallogr F Struct Biol Commun. 2
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes histidine-222 to glutamine
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71591-plasmid-map-sequence-id-130911

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  26. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Richard Murray
    Article
    Kapasiawala et al bioRxiv 2023.03.22.533877
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227645-plasmid-map-sequence-id-452394

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  30. pJK278

    Plasmid
    #71134
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to asparagine; additional N-…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71134-plasmid-map-sequence-id-131033

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  31. pJK130

    Plasmid
    #71129
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed valine-319 to phenylalanine
    Promoter
    T7
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71129-plasmid-map-sequence-id-131030

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  32. pJK591

    Plasmid
    #71589
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine and aspartate-177 …
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71589-plasmid-map-sequence-id-130927

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  35. pJK173

    Plasmid
    #71831
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71831-plasmid-map-sequence-id-131049

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  36. pJK320

    Plasmid
    #71144
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to serine; additional N-term…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71144-plasmid-map-sequence-id-131043

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  37. pJK319

    Plasmid
    #71143
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to alanine; additional N-ter…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71143-plasmid-map-sequence-id-131042

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  38. pJK318

    Plasmid
    #71142
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to glutamine; additional N-t…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71142-plasmid-map-sequence-id-131041

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  39. pJK315

    Plasmid
    #71141
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to glycine; additional N-ter…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71141-plasmid-map-sequence-id-131040

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  40. pJK314

    Plasmid
    #71140
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to valine; additional N-term…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71140-plasmid-map-sequence-id-131039

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  41. pJK174

    Plasmid
    #71835
    Purpose
    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7 and endogenous
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71835-plasmid-map-sequence-id-131052

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  42. pJK279

    Plasmid
    #71135
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to aspartate; additional N-t…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71135-plasmid-map-sequence-id-131034

    Enlarge

  43. pJK283

    Plasmid
    #71139
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine; additional N-…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71139-plasmid-map-sequence-id-131038

    Enlarge

  44. pJK282

    Plasmid
    #71138
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate; additional N-t…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71138-plasmid-map-sequence-id-131037

    Enlarge

  45. pJK281

    Plasmid
    #71137
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to phenylalanine; additional…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71137-plasmid-map-sequence-id-131036

    Enlarge

  46. pJK280

    Plasmid
    #71136
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to phenylalanine; additional…
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71136-plasmid-map-sequence-id-131035

    Enlarge

  47. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

    Enlarge

  48. IgK-IFN-luc

    Plasmid
    #14886
    Purpose
    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
    Depositor
    David Baltimore
    Article
    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.
    Insert
    IgK-IFN (IFNB1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    14886-plasmid-map-sequence-id-238489

    Enlarge

  50. pBA-mCherry-EGFP-PIM

    Plasmid
    #111758
    Purpose
    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    chicken beta-actin
    Available since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111758-plasmid-map-sequence-id-222504

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Showing: 651 - 700 of 2220 results