-
Plasmid#146571PurposeMammalian Expression of HsSmg6_817-1238DepositorInsertHsSmg6_817-1238 (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg6_1239-1419-V5-SBP-MBP_I
Plasmid#146572PurposeMammalian Expression of HsSmg6_1239-1419DepositorInsertHsSmg6_1239-1419 (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax
Plasmid#190154PurposeExpresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp3 (Vamp2 Rat)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only rat Vamp3 AA #1-81PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-IST1 N-terminal domain
Plasmid#184805PurposeBacterial expression of IST1 N-terminal domainDepositorInsertIST1 (IST1 Human)
UseTagsHis6-MBPExpressionBacterialMutationaa 1-187 onlyPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-MBP-TEV-Cas6-RT-Cas1_WT
Plasmid#186274PurposeExpresses Thiomicrospira Cas6-RT-Cas1 in E. coliDepositorInsertThiomicropira Cas6-RT-Cas1
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9-D830A/E831A
Plasmid#188085PurposeExpresses human ATG9-D830A/E831A mutant in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationD830A/E831APromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9 (692-839)
Plasmid#188092PurposeTo make monomeric ATG9 C-terminal tail for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (692-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9 (801-839)
Plasmid#188093PurposeTo make monomeric ATG9 C-terminal tail for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (801-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP SMT3 NikJC199U
Plasmid#174363PurposeExpression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C R459E
Plasmid#164024PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, R459EPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C R464E
Plasmid#164025PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, R464EPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C E485R
Plasmid#164026PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, E485RPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C E490R
Plasmid#164027PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, E490RPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C R495E
Plasmid#164028PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, R495EPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C D511K
Plasmid#164029PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, D511KPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C Y527A
Plasmid#164030PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)DepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationA452C, Y527APromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 UPA-CARD E1461R
Plasmid#164007PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)DepositorInsertNLRP1 (NLRP1 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationE1461RPromoterAvailable sinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 UPA-CARD W1460A
Plasmid#164006PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)DepositorInsertNLRP1 (NLRP1 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationW1460APromoterAvailable sinceFeb. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 UPA-CARD M1457A
Plasmid#164005PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)DepositorInsertNLRP1 (NLRP1 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationM1457APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 UPA-CARD Y1445A
Plasmid#164004PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD (dimer interface mutation)DepositorInsertNLRP1 (NLRP1 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationY1445APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N+D280N)
Plasmid#134292PurposeExpresses GK5 (isoform 2)-D30N/D280N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acids 30 and 280 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-Gly-RadA∆C46
Plasmid#128702PurposeExpresses PhoRadA intein split at residue 126 from the N-terminalDepositorInsertRadA
UseTagsHisx6-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k
Plasmid#127399PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon and W34…PromoterCMVAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k
Plasmid#127408PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsFLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, E309C …PromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k
Plasmid#127405PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon and W34…PromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Trp-eK-Flv
Plasmid#83390Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Asp-eK-Flv
Plasmid#83389Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-His-eK-Flv
Plasmid#83388Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Lys-eK-Flv
Plasmid#83387Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Leu-eK-Flv
Plasmid#83386Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Arg-eK-Flv
Plasmid#83385Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Phe-eK-Flv
Plasmid#83384Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8r
Plasmid#208318PurposeOverxpression MBP-TadA8rDepositorInsertTadA8r
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8e
Plasmid#208319PurposeOverxpression MBP-TadA8eDepositorInsertTadA8e
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only