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Plasmid#200095Purpose1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK245RPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
TXNIP-py-FLAG
Plasmid#200092PurposeTXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vectorDepositorInsertTXNIP (TXNIP Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationpy motifs mutated (aa331-334 and 375-378)PromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2h70CRET(#325)
Plasmid#184060Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Hsp70-4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAK175
Plasmid#178601PurposepAP259 derived plasmid encoding a xylose inducible BitlucOptDepositorInsertBitlucopt
UseTagsExpressionBacterialMutationPromoterPxylAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0123
Plasmid#177055PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoterDepositorInsert7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-MKP-3 C293S
Plasmid#131525PurposeExpresses MKP-3 with C293S mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Cysteine 293 to SerinePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-FOXO1 1-558
Plasmid#131531PurposeExpresses FOXO1 1-558 in mammalian cellsDepositorInsertForkhead box O1 (Foxo1 Mouse)
UseAdenoviralTagsNoExpressionMutationonly reserved amino acid 1-558PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-FOXO1 1-456
Plasmid#131532PurposeExpresses FOXO1 1-456 in mammalian cellsDepositorInsertForkhead box O1 (Foxo1 Mouse)
UseAdenoviralTagsNoExpressionMutationonly reserved amino acid 1-456PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-FOXO1 1-280
Plasmid#131534PurposeExpresses FOXO1 1-280 in mammalian cellsDepositorInsertForkhead box O1 (Foxo1 Mouse)
UseAdenoviralTagsNoExpressionMutationonly reserved amino acid 1-280PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_G252R_TEV
Plasmid#47704PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252RDepositorInsertOlfactomedin-like domain with G252R mutation (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged Glycine 252 ArgininePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_K398R_TEV
Plasmid#47701PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398RDepositorInsertOlfactomedin-like domain with K398R mutation (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged Lysine 398 to ArgininePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRARE-MBP-DEST
Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA
Plasmid#210809PurposeFor transient transfectionDepositorInsertALDOA (ALDOA Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
BglB in pET29b(+)
Plasmid#55773PurposeExpresses beta-glucosidaseDepositorInsertBeta-glucosidase B (bglB Synthetic)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG_ALDOA in pCW57_tTA_Blast
Plasmid#154210PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOADepositorInsertALDOA (ALDOA Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag HA PGC-1 alpha
Plasmid#14426DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsFlag and HAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-9xGRE
Plasmid#74736PurposeGRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elementsDepositorInsertEGFP
UseZebrafish expressionTagsExpressionMutationPromoternine GC-responsive elements (9xGRE) from the rat …Available sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
MuHKII-pGFPN3
Plasmid#21922DepositorInsertMutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …PromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
TrHKII-pGFPN3
Plasmid#21921DepositorInsertTruncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the truncated human HKII cDNA sequence (l…PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TKFC in pLJM60
Plasmid#154206PurposeLentiviral vector for stable expression of TKFCDepositorInsertTKFC (DAK Human)
UseLentiviralTagsN terminal FLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag-SIRT1 H355A
Plasmid#8439DepositorInsertSIRT1 (Sirt1 Mouse)
UseAdenoviralTagsFlagExpressionMammalianMutationHistidine 355 mutated to AlaninePromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
5K-tails-->R GLUT1-GFP
Plasmid#200099Purposehuman GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK-->R mutations of amino acids 6, 7, 451, 456,…PromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA p53, mitfa:Cas9
Plasmid#118841PurposeTargets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInserttp53 gRNA (tp53 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA
Plasmid#210806PurposeFor stably expression in cellsDepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG HDAC5 S259A/S498A
Plasmid#32216DepositorInsertHDAC5 (HDAC5 Human)
UseTagsFlagExpressionMammalianMutationSer259Ala, Ser498AlaPromoterCMVAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track HA PGC-1 alpha
Plasmid#14427DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA spred1, mitfa:Cas9
Plasmid#118843PurposeTargets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertspred1 gRNA (spred1 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Axin1_HA in pRK5
Plasmid#154220PurposeC-terminal HA-tagged AXIN1 cDNA expression vector for transfectionsDepositorInsertAXIN1 (AXIN1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
WDR59 in pRK5
Plasmid#154214PurposeUntagged WDR59 cDNA expression vector for transfectionsDepositorInsertWDR59 (WDR59 Human)
UseTagsNONEExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA
Plasmid#210812PurposeTo package adenovirus expressing wild-type aldolaseADepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only