-
Plasmid#217681PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB1 (ADGRB1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-926PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG
Plasmid#217739PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-844PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax
Plasmid#190153PurposeExpresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only AA #1-94PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG
Plasmid#217740PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL2 (ADGRL2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-830PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL2 CTF-FLAG
Plasmid#217707PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL2 (ADGRL2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-824PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
UseTagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Micro
Plasmid#60410PurposeE. coli expression of SspB MicroDepositorInsertSspB micro
UseTagsHis-MBP-TEVExpressionBacterialMutationR73Q, Y11K and A15EPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsStaufen1-T3_V
Plasmid#147744PurposeMammalian Expression of HsStaufen1-T3DepositorInsertHsStaufen1-T3 (STAU1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CfaC-MBP
Plasmid#126018PurposeBacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-CfaC-MBP
UseTags6xHis-SUMO and HA-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsStaufen1-T2_V
Plasmid#147743PurposeMammalian Expression of HsStaufen1-T2DepositorInsertHsStaufen1-T2 (STAU1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ106: pRS426-His-MBP-NlovFz2_Native
Plasmid#205253PurposeS. cerevisiae expression vector for NlovFz2 native guideDepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ45: pRS426-His-MBP-SpuFz1_Native
Plasmid#205252PurposeS. cerevisiae expression vector for SpuFz1 native guideDepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG
Plasmid#217724PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-344PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE3 CTF-FLAG
Plasmid#217691PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-338PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTF-FLAG
Plasmid#217690PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-517PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG
Plasmid#217723PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-523PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ123: pRS426-His-MBP-SpuFz1_PSP1
Plasmid#205256PurposeS. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)DepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
Plasmid#217706PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-838PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1 (WT)
Plasmid#208871PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsMBP-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ240: pRS426-His-MBP-MmeFz2_Native
Plasmid#205255PurposeS. cerevisiae expression vector for MmeFz2 native guideDepositorInsertMmeFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG81-MBP-t-M.MpeI-N374K-His
Plasmid#197985PurposeExpresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.DepositorInsertMBP-t-M.MpeI-N374K-His
UseTagsHis and MBPExpressionBacterialMutationM.MpeI-N374KPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA3 CTF-FLAG
Plasmid#217680PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA3 (ADGRA3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-737PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA2 CTF-FLAG
Plasmid#217679PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA2 (ADGRA2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-746PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ235: pRS426-His-MBP-GtFz1_Native
Plasmid#205254PurposeS. cerevisiae expression vector for GtFz1 native guideDepositorInsertGtFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-MBP-CfaN
Plasmid#126016PurposeBacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)DepositorInsertHis5-Sumo-MBP-CfaN
UseTags5xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsStaufen2-T1_V
Plasmid#147745PurposeMammalian Expression of HsStaufen2-T1DepositorInsertHsStaufen2-T1 (STAU2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ULK1 (836-1050)-MBP
Plasmid#203562PurposeMammalian expression of ULK1 (836-1050) with MBP tagDepositorInsertULK1 (ULK1 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-FLAG-LEM
Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
UseTagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only