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Lenti-sgBrip1#1/Cre

Plasmid

#193203

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgBrip1#1 (Brip1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFlt4#1/Cre

Plasmid

#193215

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#1 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgGrin2a#1/Cre

Plasmid

#193217

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Grin2a gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgGrin2a#1 (Grin2a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFam135b#1/Cre

Plasmid

#193213

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Fam135b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFam135b#1 (Fam135b Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgCtrl#2

Plasmid

#174143

Purpose

Lentiviral vector expressing a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W

Plasmid

#159290

Purpose

Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT6A (ID. A8) (KAT6A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT6A-A7)-PGKpuro2ABFP-W

Plasmid

#159289

Purpose

Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A7)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT6A (ID. A7) (KAT6A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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R26-dCas9-VPR-mNG

Plasmid

#172441

Purpose

Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

dCas9-VPR
mNeonGreen

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q)

Plasmid

#170125

Purpose

Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD(E488Q)-NES

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a core promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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gTBEv3

Plasmid

#220619

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

et al Nat Commun 15

Insert

hUNG2-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBWB424

Plasmid

#140638

Purpose

RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-mU6-BlasGO

Plasmid

#136906

Purpose

Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

BlasCyGO

Use

Lentiviral

Tags

Expression

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-mU6-Luc2GO-PGK-Neo

Plasmid

#136905

Purpose

Lentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

Luc2CyGo

Use

Lentiviral

Tags

Expression

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9-L2

Plasmid

#141038

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
gCBEv2

Plasmid

#220617

Purpose

vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

et al Nat Commun 15

Insert

hUNG2-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM41991

Plasmid

#153219

Purpose

Level M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge