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pBiT3.1-C_ARPC1A:1-370

Plasmid

#211267

Purpose

Mammalian expression of full-length human ARPC1A. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1A:M1-M370 (ARPC1A Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ARPC1B:1-372

Plasmid

#211268

Purpose

Mammalian expression of full-length human ARPC1B. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_ELP2:1-826

Plasmid

#211263

Purpose

Mammalian expression of full-length human ELP2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ELP2:M1-L826 (ELP2 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_CDC40:1-579

Plasmid

#211032

Purpose

Mammalian expression of full-length human CDC40. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CDC40:M1-D579 (CDC40 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR5:1-334

Plasmid

#211044

Purpose

Mammalian expression of full-length human WDR5. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR5:M1-C334 (WDR5 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

COPB2:M1-A597 (COPB2 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1gamma

Plasmid

#181902

Purpose

Fluorescently tagged HP1gamma

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1gamma (Cbx3 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCC336

Plasmid

#110167

Purpose

miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

Depositor

Iva Greenwald

Article

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

Phospho-acceptor sites mutated: S43E, T55E, T62E.…

Promoter

lin-31 promoter/enhancer

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCC335

Plasmid

#110166

Purpose

miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

Depositor

Iva Greenwald

Article

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

Phospho-acceptor sites mutated: S43A, T55A, T62A.…

Promoter

lin-31 promoter/enhancer

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV

Plasmid

#24828

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Plasmid

#159874

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Plasmid

#159866

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-His6

Plasmid

#212780

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-yeGFP

Plasmid

#212769

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Plasmid

#159844

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FUS/TLS-FLAGC

Plasmid

#60362

Purpose

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

Depositor

Patrick Calsou, Sébastien Britton

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

FUS (FUS Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMN20

Plasmid

#11573

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pMN19

Plasmid

#11572

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge