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Plasmid#224274PurposeCell transfection and expression of RalADepositorInsertRalA (RALA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1
Plasmid#224773PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec22b-shR
Plasmid#208358PurposeMammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22bDepositorInsertSec22b (Sec22b Rat)
UseTagsEGFPExpressionMammalianMutationfour silent mutations introduced to render shRNA …PromoterCMVAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP SV2A GFP
Plasmid#218544PurposeMamallian Expression of SV2A GFPDepositorInsertSV2A
UseTagsExpressionMammalianMutationS42FPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA4
Plasmid#164934PurposeExpresses EGFP sgRNA4 in mammalian cellsDepositorInsertsgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
T5-pAc5.1 EGFP-EcoRV
Plasmid#206481PurposeSchneider cell imagingDepositorTypeEmpty backboneUseTagsEGFPExpressionInsectMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-EGFP
Plasmid#209058PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged EGFPDepositorInsertEGFP
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-miniTurbo-EGFP
Plasmid#209059PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to EGFPDepositorInsertEGFP
UseTagsminiTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-C1-Nir3-LNS2
Plasmid#220087PurposeC-terminal PA binding domain of Nir3DepositorInsertPITPNM2 (PITPNM2 Human)
UseTagsmEGFP-GGGGSHMExpressionMammalianMutationamino acids 925-1209PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRKCZ-C1-EGFP (C-PRKCZ-C1)
Plasmid#217759PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertProtein kinase C zeta (PRKCZ Human)
UseTagsEGFPExpressionMammalianMutationC1 domain (aa 123-193)PromoterCMVAvailable sinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-PRKCZ-C1 (N-PRKCZ-C1)
Plasmid#217760PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertProtein kinase C zeta (PRKCZ Human)
UseTagsEGFPExpressionMammalianMutationC1 domain (aa 123-193)PromoterCMVAvailable sinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-RepoMan-RATA
Plasmid#184270PurposeExpresses shRNA resistant GFP-RepoMan RATA MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTags3xHA and GFPExpressionMammalianMutationV383A, F395APromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H2B-MCS
Plasmid#184041PurposeCloning of GFP-H2B fusion proteinsDepositorTypeEmpty backboneUseTagsGFP and H2BExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-T394A
Plasmid#204994PurposeExpresses shRNA resistant GFP-RepoMan T394A MutantDepositorInsertcell devision cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Threonine 394 to AlaninPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-S893D
Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-1-SAC1deltaTMD-Fis1tail
Plasmid#220077PurposeSAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green FluorophoreDepositorInsertSACM1l-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationdeltaTMDPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT74
Plasmid#218730PurposeExpresses N-terminally EGFP-tagged IFT74 in mammalian cellsDepositorInsertIFT74 (IFT74 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT46
Plasmid#218724PurposeExpresses N-terminally EGFP-tagged IFT46 in mammalian cellsDepositorInsertIFT46 (IFT46 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only