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Plasmid#123750PurposeEncodes mScarlet as a Type 3 part to be used in the MTK systemDepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
WNT11-bio-His
Plasmid#53430PurposeExpresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertWNT11 (WNT11 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm4_A
Plasmid#145883PurposeInsect Expression of DmLSm4DepositorInsertDmLSm4 (LSm-4 Fly)
UseTagsV5-6xHisExpressionInsectMutationtwo silent mutations compared to the sequence of …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S
Plasmid#98679PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5SDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887SPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ908
Plasmid#44474DepositorInsertMinimal Mos1, PuroR, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-HA-REDD1 P139A (#625)
Plasmid#99945Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationP139APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S137A (#627)
Plasmid#99943Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS137APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK2_016
Plasmid#123708PurposeEncodes truncated pCMV as a Type 2 part to be used in the MTK systemDepositorInsertpCMV-A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_017
Plasmid#123709PurposeEncodes truncated pCMV as a Type 2 part to be used in the MTK systemDepositorInsertpCMV-B
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 S166D
Plasmid#16238DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationSer166 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD38-52
Plasmid#98685PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationheptads 38-52, residues 1853-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-MESVD/TRAP(RK)-C
Plasmid#61967PurposeDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVDDepositorInsertsN-mCherry-MESVD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
TR-5'UTR-HP-GFP
Plasmid#80594PurposeExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEIS1B-pSP65
Plasmid#21019DepositorInsertMEIS1B (Meis1 Mouse)
UseIn vitro tntTagsFlagExpressionMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_002
Plasmid#123841PurposeEncodes SV40 polyadenylation signal as a Type 4b part to be used in the MTK systemDepositorInsertSV40 pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_005
Plasmid#123819PurposeEncodes Mitochondria Targeting Sequence as a Type 4a part to be used in the MTK systemDepositorInsertMTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only