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Showing: 5521 - 5560 of 7539 results

  2. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid
    #106204
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106204-plasmid-map-sequence-id-204613

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  3. pBGE3.0

    Plasmid
    #165988
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165988-plasmid-map-sequence-id-378724

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  5. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

    Plasmid
    #106205
    Purpose
    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184V + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106205-plasmid-map-sequence-id-204612

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  7. HA-hDAGLa-V5

    Plasmid
    #87674
    Purpose
    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Article
    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do
    Insert
    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    Use
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87674-plasmid-map-sequence-id-173603

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  10. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid
    #105801
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: FLAG-3C; ORF2: mClover3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105801-plasmid-map-sequence-id-203395

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  13. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid
    #106206
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A + mRuby3 fusion
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106206-plasmid-map-sequence-id-201033

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  15. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid
    #105805
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105805-plasmid-map-sequence-id-207906

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  16. pCMV-hAR-H874Y

    Plasmid
    #89087
    Purpose
    mammalian expression of human androgen receptor with prostate cancer somatic mutation H874Y from CWR22 xenograft
    Depositor
    Elizabeth Wilson
    Article
    Tan et al Mol Endocrinol. 1997 Apr;11(4):450-9. do
    Insert
    hAR-H874Y prostate cancer mutant (AR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hAR-H874Y
    Promoter
    CMV
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106202
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106202-plasmid-map-sequence-id-204609

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  20. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106178
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106178-plasmid-map-sequence-id-204208

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  23. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106189
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106189-plasmid-map-sequence-id-204217

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  27. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106203
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106203-plasmid-map-sequence-id-201030

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  29. iMb-Notch-Mosaic (IR99.40)

    Plasmid
    #99749
    Purpose
    Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes
    Depositor
    Rui Benedito
    Article
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    99749-plasmid-map-sequence-id-199304

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  31. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106183
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106183-plasmid-map-sequence-id-214796

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  32. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106195
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    GFAP
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    106195-plasmid-map-sequence-id-204275

    Enlarge

  34. pBGE1.0

    Plasmid
    #165986
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    165986-plasmid-map-sequence-id-375342

    Enlarge

  39. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106190
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106190-plasmid-map-sequence-id-204216

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Showing: 5521 - 5560 of 7539 results