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Plasmid#45590DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-EGFP tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only
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pQuantEGFP-puro-kana
Plasmid#45589DepositorTypeEmpty backboneUseCre/Lox; Dt40 cell line targetingTagsQuant-EGFP tagExpressionMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a W118S
Plasmid#40042DepositorInserthSlp4-a W118S (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationTryptophane 118 to SerinePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a V21A
Plasmid#40041DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationValine 21 to AlaninePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a SHD
Plasmid#40034DepositorInserthSlp4-a SHD (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationSHD domain (1-143)PromoterCMV promoterAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-STAG2-D299A
Plasmid#31975DepositorInserthuman STAG2 cDNA (STAG2 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationchanged Aspartic acid 299 to AlaninePromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-EGFP-ccdB
Plasmid#14303DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2-EcYRS-EGFP*
Plasmid#191118PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafishDepositorInsertMutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFP-SKL
Plasmid#183727PurposeFor exrpression of GFP targeted to peroxisomesDepositorInsertEGFP
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-EGFP-PiggyBac
Plasmid#190003PurposeExpression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.DepositorInsertMCP-EGFP
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-nonstop
Plasmid#226406PurposeExpresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.DepositorInsertEGFP without stop codon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-5.0dand5:EGFP
Plasmid#223327PurposeExpresses EGFP in Kupffer's vesicle cells in zebrafish embryosDepositorInsert5.0kb dand5 promoter (dand5 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 1.0_crEGFP
Plasmid#224790PurposeLPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selectionDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 2.0_crEGFP
Plasmid#224793PurposeLPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.DepositorInsertscrEGFP
Triplex stablier
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.mEGFP-CBX8
Plasmid#224710PurposeExpresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsmEGFPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-PERK(LD)-EGFP
Plasmid#225680PurposeUsed to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-CC
Plasmid#225476PurposeExpress mEGFP-GAPDH-CC fusionDepositorInsertGAPDH fused with CC2 domain of ROCK2 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-UGCG-EGFP
Plasmid#225878PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertionDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEW-pEF1A-inactEGFP
Plasmid#212009PurposeCleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transductionDepositorArticleInsertInactivated eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterpEF1aAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rap1A(WT)
Plasmid#224276PurposeCell transfection and expression of Rap1ADepositorInsertRap1A (RAP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only