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pRS406-CMV-lacI-3FLAG

Plasmid

#83410

Purpose

Expression of lacI in yeast

Depositor

Toshi Tsukiyama

Article

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

lacI repressor

Use

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

Promoter

CMV

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-NLS-Smad2

Plasmid

#14931

Depositor

Joan Massague

Article

Lo et al Nat Cell Biol. 1999 Dec . 1(8):472-8.

Insert

NLS-Smad2 (SMAD2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

full-length Smad2 is fused to a nuclear-localizat…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PtenC124S-Puro

Plasmid

#135677

Purpose

Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

C124S

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV mTurquoise2-shadowG tandem

Plasmid

#157774

Purpose

Produces a fusion between mTurquoise2 and shadowG. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Use

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA

Plasmid

#113896

Purpose

Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Actin

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

GENIE Project, Douglas Kim

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-oB ONE-GO

Plasmid

#189733

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oB ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 C716A

Plasmid

#11747

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C716A: catalytically inactive

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pRC/CMV-TYK2-VSV

Plasmid

#139344

Purpose

Expression of TYK2 protein

Depositor

Sandra Pellegrini

Article

Li et al PLoS One. 2020 Jan 21;15(1):e0225289. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hATG5 WT

Plasmid

#111803

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIH CMV/TO DEST (w382-1)

Plasmid

#17394

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-VPR-L1-CjCas9 V-D

Plasmid

#169913

Purpose

Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Jeff Wrana, Joan Massague

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-Sun

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116A

Plasmid

#123104

Purpose

Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-puro-2A-BLM

Plasmid

#127641

Purpose

Over-expression of human BLM

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

BLM (BLM Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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