-
Plasmid#198526PurposeExpression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)DepositorInsertFTS (AKTIP Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ε
Plasmid#197261PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-4 ε (AP4E1 Human)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitutions in codons 905, 1129 and 1137PromoterADH1Available sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9-D830A/E831A
Plasmid#188085PurposeExpresses human ATG9-D830A/E831A mutant in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationD830A/E831APromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (801-839)
Plasmid#188089PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (801-839)PromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9 (692-839)
Plasmid#188092PurposeTo make monomeric ATG9 C-terminal tail for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (692-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (723-839)
Plasmid#188088PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (723-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9 (801-839)
Plasmid#188093PurposeTo make monomeric ATG9 C-terminal tail for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (801-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (828-839)
Plasmid#188090PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (828-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAM207A_pcDNA6.2/EmGFP-Bsd
Plasmid#176950PurposeMammalian expression vector encoding FAM207A and EmGFP-BsdDepositorInsertFAM207A (C21orf70 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgAgo2_Nterm
Plasmid#170920PurposeExpresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal taggingDepositorInsertsgAgo2 (Ago2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pLV Cdipt-V5
Plasmid#175144PurposeLentiviral expression of mouse Cdipt-V5DepositorInsertCdipt (Cdipt Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b
Plasmid#109867PurposeProtein expression and purification of LRP5_Ldl-recept-bDepositorInsertLRP5_Ldl-recept-b (LRP5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b
Plasmid#109785PurposeProtein expression and purification of LRP5_Ldl-recept-bDepositorInsertLRP5_Ldl-recept-b (LRP5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)
Plasmid#156412PurposeGateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancerDepositorInsertmurine Dll4 F2- ETS site B/ site #2, 8X
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
Opto-GPR162
Plasmid#106056PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR162; Opto-GPR162; E10)DepositorInsertOpto-GPR162 (GPR162 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-AGO2 ts3
Plasmid#115855PurposeAGO2 knockdownDepositorInsertAGO2 shRNA (AGO2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CASK-1/1
Plasmid#91502PurposeProtein expression and purification of human SH3 domain construct CASK-1/1DepositorInsertCASK-1/1 (CASK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only