-
Plasmid#103690PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-630 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-630 target (MIR630 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-633
Plasmid#103691PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-633 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-633 target (MIR633 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-135b-5p
Plasmid#103229PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-135b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-135b-5p target (MIR135B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Cx26-7
Plasmid#58007PurposeLocalization: Gap Junctions, Excitation: 555, Emission: 584DepositorInsertCx26 (Gjb2 Rat)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LSB-hsa-miR-30a-5p
Plasmid#103410PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30a-5p target (MIR30A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab11 DN
Plasmid#37712DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS25N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab32 WT
Plasmid#37738DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
LLP338 pEF1a-DACH1-1-SunTag
Plasmid#100942PurposeTALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO geneDepositorInsertTALE target sequence for DACH1, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Ptk70-SH2
Plasmid#46473DepositorInsertPtk70 (Srms Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 123-236PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Rin2-SH2
Plasmid#46475DepositorInsertRin2 (RIN2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 94-193PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Bks-SH2
Plasmid#46406DepositorInsertBks (STAP2 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 141-266PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nap4-SH2
Plasmid#46456DepositorInsertNap4 (SOCS7 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 395-514PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nsp1-SH2
Plasmid#46459DepositorInsertNsp1 (SH2D3A Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 3-119PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slnk-SH2
Plasmid#46502DepositorInsertSlnk (Sh2d6 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 199-323PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs7-SH2
Plasmid#46509DepositorInsertSocs7 (SOCS4 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 280-386PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-luciferase
Plasmid#113354Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG415-ADH1p-HA-rap1-K9R
Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3NR
Plasmid#84533PurposeThis plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,117,118)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3CR
Plasmid#84534PurposeThis plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(462,463,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K9R
Plasmid#84535PurposeThis plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK651RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-AGMpygTRIM5-FOS
Plasmid#79064PurposeExpress African green monkey pygerythrus TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(African green monkey pygerythrus)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab11 CA
Plasmid#53472DepositorInsertRab11 (Rab11 Fly)
UseTagsYFPExpressionInsectMutationQ70LPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC E254D
Plasmid#100033PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.DepositorInsertRhoGC E254D
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationE254DPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab32 DN
Plasmid#37739DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT33N (dominant negative)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)
Plasmid#79036PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS1-mutant RhoA 3'UTR
Plasmid#26090DepositorInsertmutant RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationBoth miR-31 binding sites in 3’ UTR mutagenized. …PromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab32 CA
Plasmid#37740DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ79L (constitutive active)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_BFP
Plasmid#45302DepositorInsertBFP
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBa.FRB-3myc-KIF13AS1371A tail 361-1749
Plasmid#224411PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsFRB-3mycExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZU-LAMP1-mRuby3
Plasmid#222586PurposeStable expression of LAMP1-mRuby3 in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-LC3
Plasmid#221738PurposeStable expression of mRuby3-LC3 in mammalian cellsDepositorInsertLC3B (Map1lc3b Rat)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Cterm_His
Plasmid#220487Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Nterm_His
Plasmid#220482Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
95.a_pSTC0-zeo
Plasmid#202334PurposeDesign 95.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert95.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
90.a_pSTC0-zeo
Plasmid#202336PurposeDesign 90.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert90.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
80.a_pSTC0-zeo
Plasmid#202338PurposeDesign 80.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert80.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
70.a_pSTC0-zeo
Plasmid#202340PurposeDesign 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert70.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
50.a_pSTC0-zeo
Plasmid#202342PurposeDesign 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert50.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
98.a_pSTC0-zeo
Plasmid#202332PurposeDesign 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert98.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-GFP
Plasmid#207018PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTPI1-Dsred-URA3
Plasmid#207017PurposeExpresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).DepositorInsertDsRed-Express 2
UseTagsExpressionYeastMutationPromoterpTPI1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
UseTags9xMycExpressionYeastMutationPromoterpADH1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-Y387A-GFP
Plasmid#207022PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationY387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A-GFP
Plasmid#207021PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-I375A-GFP
Plasmid#207020PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationI375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only