-
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-p38alpha
Plasmid#47575PurposeBacterial expression plasmid for GST-3xFlag-tagged p38alphaDepositorInsertp38alpha (Mapk14 Mouse)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pICE
Plasmid#46960PurposePlasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cn-VA-NHEJ1-WT
Plasmid#141339PurposeLentiviral vector for expression of human NHEJ1-WT in mammalian cellsDepositorInsertNHEJ1 (NHEJ1 Human)
UseLentiviralTagsVA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mNeonGreen
Plasmid#137802PurposeVisualization of microtubulesDepositorArticleInsertEnsconsin microtubule binding domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMX-hTAF11
Plasmid#44341DepositorInsertTAF11 (TAF11 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-HA-hTAF5
Plasmid#17984DepositorInsertTAF5 (TAF5 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
EF1a_mCherry_P2A_Hygro
Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionTagsExpressionWormMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMal-E6LLO
Plasmid#51281PurposeExpresses a fibronectin fusion with listeriolysin targeting EGFR in bacteriaDepositorInsertE6LLO
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-wt
Plasmid#110214PurposeExpression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionMammalianMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB505
Plasmid#74847PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-CeNL_pcDNA3
Plasmid#89513PurposeCyan color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertCeNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3376
Plasmid#144852PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX (TOX Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCherry-FRB
Plasmid#25920DepositorInsertFRB (MTOR Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro HA Brca1
Plasmid#14999DepositorInsertBrca1 (BRCA1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF2972
Plasmid#144448PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR1H4 (NR1H4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635DepositorTypeEmpty backboneUseTags6xHis and mKate1.31ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet-1_mNeongreen
Plasmid#182413PurposeBacterial expression of mNeongreenDepositorInsertmNeongreen
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEThT-CAHS3
Plasmid#90028PurposeTo express His-tagged CAHS3 in bacteriaDepositorInsertCAHS3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.011
Plasmid#108529PurposeTetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.DepositorInsertTetR + PTet*-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21(+)Tm9D8* TrpB
Plasmid#207517PurposeExpression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serineDepositorInsertTryptophan synthase β-subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEThT-CAHS1
Plasmid#90027PurposeTo express His-tagged CAHS1 in bacteriaDepositorInsertCAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_MARV
Plasmid#135471PurposeEncodes Marburg virus VP35 gene under control of the AG promoterDepositorInsertVP35
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag beta-2-adrenergic-receptor
Plasmid#14697DepositorInsertB2AR (ADRB2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hTXLNA-HA
Plasmid#165472PurposeHuman TXLNA mammalian expression vector with C-terminal HA tag.DepositorInserttaxilin alpha (TXLNA Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_PURO
Plasmid#155295PurposeLentiviral vector to generate hACE2 stable expressing cell lineDepositorInserthACE2 (ACE2 Human)
UseLentiviralTagsC9ExpressionMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_BIRC6
Plasmid#197974PurposeEntry clone for wt BIRC6DepositorInsertBIRC6 (BIRC6 Human)
UseOtherTagsExpressionMutationL1332VPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9_GFP
Plasmid#64709PurposeCo-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cellsDepositorInsertSaCas9-2A-GFP
UseCRISPRTags2A-GFP and 3xFLAG-SV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorInsertSAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_NP_MARV
Plasmid#135470PurposeEncoding Marburg virus NP gene under control of the AG promoterDepositorInsertnucleoprotein (NP)
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_MARV
Plasmid#135472PurposeMammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoterDepositorInsertVP30
UseTagsExpressionMammalianMutationNonePromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_MARV
Plasmid#135473PurposeMammalian expression vector encoding Marburg virus L gene under control of the AG promoterDepositorInsertL gene
UseTagsExpressionMammalianMutationsilent mutation (C to T) at position 7861; insert…PromoterAGAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2,0_3M5M_eGFP
Plasmid#135474PurposeEncodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsertMarburg virus minigenome, eGFP reporter
UseTagsExpressionMammalianMutationNonePromoterT7Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE
Plasmid#133282PurposeLentiviral expression of a peroxisomal eGFPDepositorInsertPeroxisome targeting signal 1
UseLentiviralTagsPeroxisome targeting signal 1ExpressionMammalianMutationPromoterNoneAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-ReNL_pcDNA3
Plasmid#89533PurposeRed color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertReNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3283
Plasmid#144759PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXA5 (HOXA5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-BACE1-mBFP
Plasmid#196697PurposeExpresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertBeta-secretase 1 (BACE1 Human)
UseTagsHA and mBFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pCG-Hc∆18
Plasmid#84817PurposeMeasles virus hemagglutinin expressing plasmidDepositorInsertMeasles virus H glycoprotein
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-ABCC8-2
Plasmid#135916PurposeEncodes ABCC8 exon 2 wild type for the analysis of splicing variantsDepositorInsertATP-binding Cassette subfamily C member 8 (ABCC8 Human)
UseExon trappingTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.ffLuciferase.SV40
Plasmid#105532PurposeAAV expression of firefly luciferase from CMV promoterDepositorHas ServiceAAV1 and AAV8InsertLuciferase
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
BA31_pU.CAG.SaCas9-D10A.rBGpA
Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSico
Plasmid#11578DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLEXSY-hyg2.1_LtIFT43_Flag
Plasmid#194433PurposeExpression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolaeDepositorInsertIntraflagellar transport complex subunit 43
UseLeishmania tarentolae expressionTags3X Flag and LinkerExpressionMutationPromoterL.donovani promoter elementsAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only