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Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Neisseria meningiditis, Human, Synthetic)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.A184S
Plasmid#106180PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV5InsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterhSynapsinAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD2_miniluc_newP7
Plasmid#174105Purposeadding the mini luciferase and mini promoter to the MPRA lenti constructDepositorInsertmini luciferase mini-promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam hum LaminB1
Plasmid#182671PurposeEukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.DepositorInsertLamin B1 (LMNB1 Human)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-IDH1
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorInsertisocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDM-SARS-CoV-2 Δ18
Plasmid#164434PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SB
Plasmid#177936PurposeExpresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD
Plasmid#171238PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TH0901-pOCC177-FUS-wt_opt(Nhe)
Plasmid#221885PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSV39-ugi
Plasmid#186556PurposeBroad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)DepositorInsertugi
UseTagsExpressionBacterialMutationPromoterLacUV5Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD
Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221894PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertEWSR1 (EWSR1 Human)
UseTags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184S
Plasmid#106174PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1 and AAV9InsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Notch1 intracellular domain-pcw107-V5
Plasmid#64622Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertNOTCH1 (NOTCH1 Human)
UseLentiviralTagsV5ExpressionMutationintracellular domain *PromoterPGKAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_wt
Plasmid#88871Purposeinducible expression of GST-DDX5 in bacteriaDepositorInsertDDX5 (DDX5 Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD-DBD
Plasmid#171236PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1 (S218D,S222D)-pcw107
Plasmid#64604Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsExpressionMutationS218D, S222DPromoterPGKAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hGPR56-IRES-GFP
Plasmid#52297PurposeExpresses human GPR56 and GFP in mammalian cells.DepositorInsertHuman GPR56 coding sequence (ADGRG1 Human)
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SBECN1W
Plasmid#107937PurposeAdeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neuronsDepositorInsertmouse Beclin-1 (BECN1) (Becn1 Mouse)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV5
Plasmid#74984PurposeConstitutive ETV5 ORF (codon optimized), blasticidin selectionDepositorUseTagsExpressionMammalianMutationCodon optimized (synonymous)PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18
Plasmid#164436PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Notch3 intracellular domain-pcw107-V5
Plasmid#64623Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertNOTCH3 (NOTCH3 Human)
UseLentiviralTagsV5ExpressionMutationintracellular domain *PromoterPGKAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Control-Mosaic (SO274)
Plasmid#99751PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteinsDepositorInsertHIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
iMb2-Control-Mosaic (SO273)
Plasmid#99750PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteinsDepositorInsertMb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MEKDD
Plasmid#31202DepositorInsertMAPKK1 (MAP2K1 Human)
UseGateway donor vectorTagsExpressionMutationS218D S222DPromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
TGFbetaR1 (T204D)-pcw107-V5
Plasmid#64629Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertTGFBR1 (transcript variant 1) (TGFBR1 Human)
UseLentiviralTagsV5ExpressionMutationT204DPromoterPGKAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA
Plasmid#168305Purposemediated knock-in of sgRNA precursorDepositorInsertSaKKH-EGFP-U6-sgRNA
UseAAVTagsExpressionMammalianMutationPromoterEF1alpha, CBhAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG
Plasmid#154000PurposeExpress CasRx for AAV packageDepositorInsertCasRx
UseCRISPRTagsFLAGExpressionMutationPromoterGFAPAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S
Plasmid#106177PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-d
Plasmid#108541PurposeExpresses human PCDH7 transcript variant d in mammalian cellsDepositorInsertPCDH7 transcript variant d (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.A184S
Plasmid#106201PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107
Plasmid#64615Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMThy1.1_hDDX5_FL
Plasmid#88873Purposeconstitutive expression of DDX5 in mammalian cellsDepositorInsertDDX5 (DDX5 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2
Plasmid#164435PurposeEncodes SARS-CoV-2 Spike Protein for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Notch1 intracellular domain-pcw107
Plasmid#64621Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertNOTCH1 (NOTCH1 Human)
UseLentiviralTagsExpressionMutationintracellular domain *PromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHDM-SARS-CoV-2
Plasmid#164433PurposeEncodes SARS-CoV-2 Spike Protein for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only