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Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
UseTags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_HuD
Plasmid#65763Purposeexpresses GFP-tagged HuD in mammalian cellsDepositorInsertELAVL4 (ELAVL4 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP
Plasmid#195503PurposeSOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locusDepositorInsertSOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
UseCRISPR; Donor vectorTagsT2A-H2B-mCitrineExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pimEJ5GFP
Plasmid#44026DepositorInsertEJ5GFP egfp-based chromosomal break reporter
UseTagsExpressionMammalianMutationpCAGGS promoter and eGFP separated by pgkPURO cas…PromoterpCAGGSAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-3XKD
Plasmid#25049DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSKI074
Plasmid#11571DepositorInsertCaMV 35S enhancers
UseActivation taggingTagsExpressionPlantMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-RAMP4
Plasmid#201929PurposePiggyBac Vector expressing KEIMA-RAMP4DepositorInsertRAMP4 (SERP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_WT
Plasmid#81923PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-TO-Vpr-mRuby3-IN
Plasmid#167584PurposeFor producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3DepositorInsertVpr-mRuby3-IN
UseTagsVpr-mRuby3ExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-PhoCl-mCherry
Plasmid#87691PurposeExpress NLS-PhoCl-mCherry in mammalian cellsDepositorInsertNLS-PhoCl-mCherry
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
UseTagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-H1047R
Plasmid#116500PurposeLentiviral expression of PIK3CA H1047RDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationH1047RPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kras (G12V)-pcw107
Plasmid#64602Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0679
Plasmid#149689PurposeBacterial expression of SENP_EuH proteaseDepositorInsertSENP_EuH protease
UseTagsHis14-tag and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-FbxO5-pcDNA3.1-
Plasmid#52509Purposeexpresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertFbxO5 (FBXO5 Human, FbxO5)
UseTagsFLAG-HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fNPY-GFP
Plasmid#22912DepositorInsertfugu neuropeptide Y promoter driving GFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CIRBP
Plasmid#11607DepositorInsertCold Inducible RNA Binding Protein (CIRBP Human)
UseTagsHAExpressionMammalianMutationno mutationsPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only