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Showing: 2741 - 2760 of 16003 results

  1. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Use
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    170757-plasmid-map-sequence-id-334720

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  9. pENTR4 CDS A. thaliana PARP1

    Plasmid
    #175818
    Purpose
    pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Insert
    PARP1 (PARP1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    one silent mutation, annotated in GenBank file
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
    175818-plasmid-map-sequence-id-346172

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  10. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

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  11. pSMPUW_6.10_M3_Ef1a-HA-gateway-SV40

    Plasmid
    #128446
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, and SV40 promoter
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, and SV40 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128446-plasmid-map-sequence-id-270525

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  12. pSMPUW_6.11_M3_Ef1a-gateway-HA-SV40

    Plasmid
    #128447
    Purpose
    lentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoter
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, C-terminal HA tag, and SV40 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128447-plasmid-map-sequence-id-256451

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  13. pSMPUW_8.2_M3_CAMKIIA-gateway-V5-SV40

    Plasmid
    #128462
    Purpose
    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, and SV40 promoter
    Depositor
    Lindy Barrett
    Insert
    CAMKIIA promoter, C-terminal V5 tag, and SV40 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128462-plasmid-map-sequence-id-270527

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  17. MCS-BioID2-HA pBabe-puro

    Plasmid
    #120308
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    -BioID2-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120308-plasmid-map-sequence-id-239324

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  18. His-GST-MPK6

    Plasmid
    #215170
    Purpose
    Encodes the glutathionine S-transferase with N-terminal His tag and fused to the N-terminus of a mitogen-activated protein kinase 6 from Arabidopsis thaliana
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    His-GST-MPK6
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Availability
    Academic Institutions and Nonprofits only
    215170-plasmid-map-sequence-id-430166

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Showing: 2741 - 2760 of 16003 results