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Plasmid#23999DepositorInsertNR1 (Grin1 Rat)
UseTagsNR1 signal sequence (aa1-20) and SEP (supereclipt…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W
Plasmid#67982PurposeCas9 activity reporter with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY-Gal∆DD (B1013)
Plasmid#92035PurposeExpresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRESDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationFusion of plant CRY2 to Gal4 binding domain (AA1-…PromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRD215
Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Phi3-AURKB-PGK-mCherry
Plasmid#153316PurposeExpresses AURKB and mCherry in mammalian cells.DepositorInsertAURKB (AURKB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest
Plasmid#79770PurposeStaging plasmid for use in DIP transpositionDepositorTypeEmpty backboneUseSynthetic Biology; Staging plasmid for transposit…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGreen_GUS_competitor
Plasmid#55208PurposeGUS-based microRNA target competitor used for testing function as target mimicryDepositorInsertbeta-glucuronidase
UsePlant expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir
Plasmid#90004PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]DepositorInsertsFKBP-linker-Myc-NBirA*
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG17-mCherry-HOTag3
Plasmid#190024PurposeTransiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.DepositorInsertLaG17 CluMPS
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only