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Showing: 2721 - 2740 of 72239 results

  6. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  8. pAAV-TRE-DIO-GCaMP6s

    Plasmid
    #183809
    Purpose
    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene TetO promoter-DIO/flex-GCaMP6
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183809-plasmid-map-sequence-id-366066

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  11. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid
    #121966
    Purpose
    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    scFv-GCN4
    Use
    Lentiviral
    Tags
    superfold GFP-GB1-NLS-iLID
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121966-plasmid-map-sequence-id-236121

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  12. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  15. pcDNA 3.3 HA-hTSPAN12

    Plasmid
    #115784
    Purpose
    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.
    Depositor
    Harald Junge
    Article
    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.
    Insert
    TSPAN12 (TSPAN12 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    multiple silent mutations introduce restriction s…
    Promoter
    CMV
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    115784-plasmid-map-sequence-id-224023

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Showing: 2721 - 2740 of 72239 results