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Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRTagsExpressionWormMutationPromoterPhsp-16.48Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)
Plasmid#159888PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-1 (w ATG)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)
Plasmid#159889PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-2(no_start)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc-ÂHis-ÂzfKitlgb
Plasmid#140293PurposeExpress soluble His-tagged zebrafish Kitlgb in insect cellsDepositorInsertKitlgb (kitlgb Zebrafish)
UseTags6x HisExpressionInsectMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Myo 9 Avi+Flag
Plasmid#134969PurposeExpression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1DepositorInsertMyo 9 (hum-7 Nematode)
UseTagsAvi and FlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV306-ribo-Sec-APEX2
Plasmid#111701PurposeTheophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)DepositorInsertAPEX2
UseTagsV5 and mpt63 signal sequenceExpressionBacterialMutationPromoterhsp60-riboAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK039
Plasmid#105425PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK038
Plasmid#105426PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG822
Plasmid#105430PurposeSynthetic biologyDepositorInsertYFP fused to SV40 Nuclear Localisation Signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG587
Plasmid#105431PurposeSynthetic biologyDepositorInsertbeta-glucuronidase (2 introns; no ATG-Start codon)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16418
Plasmid#105404PurposeSynthetic biologyDepositorInsert6xHis-NES(Rev)-GFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16429
Plasmid#105405PurposeSynthetic biologyDepositorInsert6xHis-NES(PKI)-GFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG317
Plasmid#105407PurposeSynthetic biologyDepositorInsert4xmyc tag fused to Twin-Strep tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG659
Plasmid#105410PurposeSynthetic biologyDepositorInsertLuciferase CDS fused to 3xHA tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG643
Plasmid#105411PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI037
Plasmid#105413PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor) fused to mEGFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI041
Plasmid#105414PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only